摘要
目的 探讨转化生长因子 β1 (TGF β1 )蛋白及TGF β1 mRNA在慢性胰腺炎发病过程中的作用及意义。方法 通过制作犬慢性胰腺炎模型 ,采用免疫组化SP法检测TGF β1 及原位杂交 (ISH )、逆转录—聚合酶链反应 (RT PCR )法检测TGF β1 mRNA在慢性胰腺炎形成过程不同时期组织中的表达和分布。结果 TGF β1 及TGF β1 mRNA主要表达于间质的纤维组织 ,成纤维细胞、巨噬细胞及血管内皮细胞 ,且在慢性胰腺炎形成过程的表达较强而持久。结论 TGF β1 持续性高表达是造成慢性胰腺炎形成过程中成纤维细胞增殖旺盛、细胞外基质过度沉积及胰腺组织进行性纤维化的重要因素。
Objective To study the effect and significance of transforming growth factor-β_1(TGF-β_1), and TGF-β_1mRNA in the pathogenesis of chronic pancreatitis. Methods The expression and distribution of TGF-β_1, and TGF-β_1mRNA in the pancreatic tissue in different stage of the pathogenesis of chronic pancreatitis were studied with immunohistochemical SP staining, in situ hybridization,and reverse transcription-polymerase chain reaction on the canine model of chronic pancreatitis . Results The Expression of TGF-β_1 and TGF-β_1mRNA were found in fibrotic tissues, fibroblasts, macrophages and endothelial cells of blood vessels.The expression of TGF-β_1 and TGF-β_1mRNA were high and lasting in the pathogenesis of chronic pancreatitis. Conclusions High expression of TGF-β_1 is closely related to the fibroblast proliferating activity, extracellular matrix overdeposition and proceeding fibrosis of pancreas.
出处
《中国普通外科杂志》
CAS
CSCD
2002年第10期612-615,共4页
China Journal of General Surgery
