摘要
目的:建立一种测定人体血浆中扑尔敏浓度的高效液相色谱法。方法:取人体血浆1.5 mL,加内标右美沙芬后,用三氯甲烷提取,取其有机相于60℃水浴挥干,剩余物加流动相复溶,并用乙酸乙酯洗涤,取水相20μL进样。流动相为0.025mol·L^(-1)磷酸二氢铵(用磷酸调pH为3.5)-乙腈(70:30),色谱柱为Diamonsil ODS C_(18)柱(5μm,4.6mm×150mm),检测波长为200 nm,流速为1.0mL·min^(-1),柱温为室温。结果:本方法线性范围为0.5～16ng·mL^(-1),,r=0.998 7,最低检测限为0.25ng·mL^(-1),方法回收率为96.6％～108.0％,日内RSD为4.1％～5.9％,日间RSD为4.6％～5.9％。结论:本方法灵敏度高,特异性强,重现性好,可用于人体血浆中扑尔敏浓度的测定。
Objective: A RP - HPLC method was developed for the determination of chlorpheniramine in human plasma. Method: 1. 5 mL plasma sample was extracted with chloroform and the organic phase was evaporated to dryness at 60℃. The residues was redissolved in mobile phase and cleaned with ethyl acetate. Chlorpheniramine and dextromethorphan (internal standard) was analyzed on diamonsil ODS C18 column (5μm, 4.6 mm × 150 mm) at ambient temperature and detected at 200 nm. The mobile phase was 0. 025 mol · L-1 NH4H2PO4 (pH = 3.5, adjusted with H3PO4) - acetonitrile (70:30). The flow rate was 1.0 mL · min-1. Results: The linear range was 0. 5 - 16. 0 ng· mL-1 , r = 0. 9987. The limit of determination was 0. 25 ng · mL-1 . The method recovery was 96. 6% - 108. 0% . Within - day precision ( RSD) was 4. 1%- 5. 9% , between - day precision (RSD) was 4. 6% -5.9%. Conclusion: The method was sensitive, specific and reproducible for the determination of chlorpheniramine concentration in human plasma.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2002年第6期443-445,共3页
Chinese Journal of Pharmaceutical Analysis
