摘要
利用真空冷冻干燥法对家兔角膜进行冻干贮藏·给出了真空冷冻干燥角膜工艺,主要是对角膜进行四步法冷平衡,两步法速率降温,采用变幅值、变周期循环压力法实现干燥室内压力的调节·对按该工艺冻干的兔角膜进行物理法检测,均符合检测标准;进行台盼蓝、茜素红联合染色法检测,内皮细胞呈六角形镶嵌排列;进行透射电镜、扫描电镜检测,细胞微观结构完好·检测结果表明冻干角膜具有活性,按照此工艺能够冻干出适合贮藏、再植的活性角膜·对真空冷冻干燥法贮藏的兔角膜进行了移植实验,手术成功。
?The rabbit corneas were preserved by vacuum freezedrying and the freezedrying process of corneas was presented. The freezedrying process mainly consists of the fourstep cold balance, twostep temperature drop and the pressure modulation in drying chamber by diversification cyclic pressure. The freezedried corneas were checked by physics method satisfied the examination standard. The endothelium of the freezedried corneas appear normally in morphology by the trypan blue and alizarin red; The endothelium ultrastructure of the freezedried corneas were uninjured by the transmission eletron microscope and the scanning electron microscope. The viability of the freezedrying corneas was proved by the examination methods. According to the freezedrying process, the rabbit corneas satisfies the need of cornea transplant and could be produced. The freezedrying rabbit corneas were used on animal experiment for keratoplasty in rabbit eyes, and the grafts remained clear postkeratoplasty. The vacuum freezedrying preserved corneas might be used for keratoplasty.
出处
《东北大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2002年第11期1066-1069,共4页
Journal of Northeastern University(Natural Science)
基金
国家自然科学基金资助项目(59876008)
关键词
角膜
真空冷冻干燥
冻干工艺
活性检测
角膜移植
cornea
vacuum freezedrying
freezedrying process
viability examination
keratoplasty
