摘要
目的 探讨人重组生长激素正性肌力作用的机制。方法 利用Langendorff模型以及膜片钳技术研究心肌细胞内向钙电流。结果 受到人重组生长激素作用10min后,动作电位平台期延长,APD50、APD90分别由261.14±15.42ms至354.65±31.22ms和311.23±13.7至401.33±18.13ms。最大内向峰电流(Ica)由 2.03±0.09升至2.85±0.11PA,增加约40％。结论 人重组生长激素延长动作电位平台期,促进正常豚鼠心肌细胞L-Ca2+通道开放。
Objective To explore the effect of recombinant human hormone on action potential of ventricular muscle cell and calcium channel. Methods Study the action potential and influx calcium ion current of ventricular muscle cells of guinea pigs by using Langendorff model and patch - clamp technique. Results Ten minutes after the administration of recombinant human growth hormone, the plateau of action potential prolonged significantly. The APD50 and APD90 increased from 261. 14 ± 15.42 and 311. 23 ± 13. 7ms to 354.65 ± 31.22 and 401.33 ± 18.13ms respectively. The peak calcium ion current increased by about 40% , from 2.03 ± 0.09 to 2.85 ± 0.11 PA. Conclusion Recombinant human growth hormone prolonged the plateau of action potential and promoted the opening of calcium channel.
出处
《中国生物制品学杂志》
CAS
CSCD
2002年第6期350-352,共3页
Chinese Journal of Biologicals
基金
吉林省科委资助
吉科合字第990581-2
关键词
人重组生长激素
豚鼠
心室肌细胞
动作电位
钙通道
作用
Recombinant human growth hormone Patch - clamp technique Calcium channel Action potential
