摘要
目的本研究拟通过Langendorff模型研究远端缺血预适应减低炎症反应后,是否有利于神经再生。方法选取SD大鼠,随机分为远端缺血预适应组和对照组,选取同龄SD大鼠作为Sham组。通过间断钳夹股动脉进行远端缺血预适应。取出心脏常规建立Langendorff,分别在恢复灌注后1、3、6小时取材。通过Western Blot、免疫组化和ELISA等方法进行神经再生检测。结果炎症抑制组(远端缺血预适应组)炎症明显得到抑制,新生神经增多,以交感神经为主,促神经生长因子分泌增多。结论远端缺血预适应组炎症反应较轻,新生神经增多;新生神经以交感神经为主,交感神经再生可改善供体心脏功能,远端缺血预适应能降低炎症反应。
Objective To use Langendorff model to investigate whether the reduction of inflammatory reactions by remote ischemic preconditioning is favorable for nerve regeneration. Methods SD rats were randomly divided into remote ischemic preconditioning group and control group. Remote ischemic preconditioning was achieved through interrupted occlusion of femoral artery. Heart was taken out, and Langendorff model was established using routine methods. Specimens were collected at 1, 3 and 6 hours after recovery of perfusion. Western Blot, immunohistochemistry and ELISA methods were used to detect nerve regeneration. Results In the inflammation inhibition group（remote ischemic preconditioning group）, inflammation was significantly suppressed, neogenetic nerves increased（mainly sympathetic nerves）, and the secretion of nerve growth factors increased. Conclusion In Langendorff model, remote ischemic preconditioning would be beneficial for sympathetic nerve regeneration through inflammation inhibition pathways.
出处
《中国分子心脏病学杂志》
CAS
2014年第4期1007-1011,共5页
Molecular Cardiology of China
基金
国家自然科学基金面上项目(81270302)
协和青年基金(2012-XHQN08)
阜外科技新星(2012-FWXX05)
关键词
远端缺血预适应
神经再生
心脏移植
心脏功能
Remote Ischemic Preconditioning
Nerve Regeneration
Heart Transplantation
Heart Function
