摘要
目的 :探讨EB病毒 (Epstein Barrvirus,EBV)感染在肠道淋巴瘤发病中的意义。 方法 :采用EBV的DNA原位杂交及S P法免疫组化技术 (第一抗体为EBV、CD3、CD2 0、CD43、CD45、CD45RO、CD74等 ) ,观察 2 4例肠道淋巴瘤患者 (8例肠病相关T细胞淋巴瘤、1 6例黏膜相关淋巴组织B细胞淋巴瘤 )EBV感染情况。以 2 0例慢性结肠炎作为对照。结果 :患者年龄 2 1～ 92岁 (平均 52 8岁 ) ,男女之比 3 8∶1。临床上均以腹痛、腹胀或便血就诊。组织病理学 :T细胞淋巴瘤细胞多形性 ,核大 ,不规则 ,嗜血管性及大片坏死 ;B细胞淋巴瘤细胞中等大小 ,多呈圆形、椭圆形 ,胞质较少淡染 ,核稍大 ,核分裂象多见 ,可见“淋巴上皮病变”。 2 4例淋巴瘤中检出 (原位杂交及免疫组化 )EBV DNA 1 4例 (检出率为 58.3 % ) ,其中T细胞淋巴瘤EBV的检出率为 75 % ,B细胞淋巴瘤EBV的检出率为 50 % (P <0 0 1 )。结论
Purpose To investigate the significance of Epstein Barr virus(EBV) infection in intestinal lymphomas. Methods The status of EBV infection in 24 cases of intestinal lymphomas was observed by in situ hybridization for EBV DNA and S P immunohistochemical staining technique for EBV, CD3, CD20, CD43, CD45RO, CD74 and so on. Twenty cases of chronic colitis were used for comparison. Result Twenty four cases of intestinal lymphoma (8 cases enteropathy associated T cell lymphoma and 16 cases mucosa associated lymphoid tissue B cell lymphoma), consisting of 19 male and 5 female cases, were in 21～92 years (mean, 52 8 years). All patients presented with colic, fullness and hematochezia. Histopathologically, the tumor cells in T cell lymphoma were pleomorphic and the cytoplasm was usually abundant. The neoplastic cells took delight in the blood vessel, and there were large pieces of necrosis. The tumor cells in B cell lymphoma were medium, round and oval cell. The cytoplasm was little and nuclei division could be seen. 'The lymph epithelial lesions' could be seen in the B cell lymphoma. Fourteen cases was EBV DNA positive, consisting of 6 cases of T cell lymphoma and 8 cases of B cell lymphoma ( P <0 01). Conclusion EBV infection may be releted to the pathogenesis of intestinal lymphoma.
出处
《临床与实验病理学杂志》
CAS
CSCD
2002年第5期499-502,共4页
Chinese Journal of Clinical and Experimental Pathology
关键词
肠道淋巴瘤
EB病毒
相关性研究
原位杂交
免疫组织化学
intestinal neoplasms
lymphoma
Epstein Barr virus
in situ hybridization
immunohistochemistry
