摘要
目的 :测定西红花药材中西红花苷 1、苷 2的含量。方法 :采用反相高效液相色谱 ,以Nova PakC18( 4 μm ,150mm×3 .9mm)柱为色谱柱 ,甲醇 水 ( 55∶45)为流动相 ,测定波长为 ( 4 4 0± 1)nm。结果 :西红花苷 1的平均回收率为 99.53 % ,日内、日间RSD分别为 1.0 % ,1.6 % ;西红花苷 2的平均回收率为 99.43 % ,日内、日间RSD分别为 1.3 % ,1.8%。进口西红花商品药材中有 2种未测出或只有痕量 ,3种含量较低 (西红花苷 1为 80 .9～ 10 2 .0mg·g- 1生药 ,西红花苷 2为 44.5～ 62 .3mg·g- 1生药 ) ,而国产西红花商品药材中含量最高 (西红花苷 1为 176 .2mg·g- 1生药 ,西红花苷 2为 10 8.5mg·g- 1生药 )。结论 :建立的RP HPLC法测定不同来源西红花药材中西红花苷 1、苷 -2的含量 ,操作简便 ,结果准确。
OBJECTIVE To determine the content of crocusⅠ and crocusⅡ in Crocus sativus L. of domestic and foreign available Crocus sativus L. samples.METHODS CrocusⅠ and crocusⅡ were separated on Nova Pak C 18 column with a mobile phase methanol water (55∶45)and detection wavelength of 440nm.RESULTS The standard curve of crocusⅠwas linear in the concentration range of 50～300 mg·L -1 ( r = 0.9999 ),the average rate was 99.53% .The intra day and inter day RSD were 1.0% and 1.6% respectively. The standard curve of crocus Ⅱ was linear in the concentration range of 30～300 mg·L -1 ( r = 0.9999 ),the average rate was 99.43% . The intra day and inter day RSD were 1.3% and 1.8% respectively. The results showed that the imported Crocus sativus L. contained no or a small amount of crocusⅠ and crocusⅡ (crocusⅠ:0～ 102.0 mg·g -1 ; crocusⅡ: 0～ 62.3 mg·g -1 ), but the domestic amounted almost crocusⅠ 176.2 mg·g -1 and crocusⅡ 108.5 mg·g -1 .CONCLUSIONS It is a simple and accurate method for the determine of crocusⅠ and crocusⅡ in Crocus sativus L.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2002年第11期654-656,共3页
Chinese Journal of Hospital Pharmacy
