摘要
目的 :优选栀子西红花总苷的吸附树脂及吸附条件。方法 :以西红花苷 1为对照品 ,紫外分光光度法测定含量 ,采用静态吸附法 ,考察大孔树脂的吸附、解吸性能 ,吸附动力学及影响吸附性能的因素。结果 :HPD系列树脂综合性能最佳 ;HPD30 0 (非极性 )、HPD4 5 0 (弱极性 )、HPD4 0 0 (中极性 )、HPD6 0 0 (强极性 )的吸附量分别为 1 0 0 .6 0 ,91 .1 5 ,1 0 0 .95 ,72 .2 7mg·g-1 ,解吸率为 84 .0 5 ,87.2 2 ,93.83,78.3% ,吸附平衡时间为 4 ,3,3,2h ;样品纯度越高吸附量越大 ,pH值小于 8及醇浓度小于 1 5 %时 ,吸附量无显著差异。结论 :HPD5 4 0 0树脂为最佳 ;吸附条件为 :提取液经醇沉处理后 ,在醇浓度为1 5 % 。
AIM: To select optimum macroporous resin and adsorption conditions of crocin in Fructus gardeniae. METHODS: The content of crocin was determined by UV spectrophotometer with crocin Ⅰ as standard. Property of adsorption and desorption, adsorption dynamics and factors affecting the adsorption were investigated by static adsorption method. RESULTS: The macroporus resin of HPD series had the best performance; the adsorption quantily of HPD300,HPD450,HPD400、HPD600 was 100.60 、 91.15 , 100.95 、 72.27mg·g -1 , desorption efficiency 84.05% , 87.22% , 93.83% , 78.38% , time of adsorption equilibrium was 4,3,3,2 hours respectively; the purer the sample, the higher the adsorption quantity was; the adsorption quantity had no remarkable difference when pH was less than 8 and concentration of ethanol was less than 1.5% . CONCLUSION: HPD400 was the optimum macroporous resin; the appropriate adsorption conditions consisted of 15% alcoholic sedimentation after extraction in neutral solution.
出处
《中成药》
CAS
CSCD
北大核心
2004年第7期532-534,共3页
Chinese Traditional Patent Medicine
关键词
大孔吸附树脂
栀子
西红花苷
吸附
macroporous resin
Fructus gardeniae
crocin
adsorption
