摘要
本文首次报道连续生产精品肝素钠的新工艺。猪小肠粘膜的提取液在用离子交换树脂吸附之前,先经过氧化除杂蛋白和脱色过程,既提高了树脂对肝素钠的吸附率,同时也减轻提取液中杂质对树脂的竞争和毒害。肝素钠从树脂上被洗脱后,再连续地进行再氧化、除酸、碱性蛋白和核酸、酒精反复沉淀等工艺过程,生产出精品肝素钠,效价为156±5u/mg,得率为1.092±0.035亿单位/1000kg粘膜,本工艺具有较大的应用价值。
This paper reports the new unbroken technological process of producing refinned heparin first. The aqueous extracts from swine intestinal mucosa are adsorped by resin (D_(264)) after deproteinization and decolouration, that improves the absorption of resin to heparin, lightens the competition and poison of impurities to resin. After crude heparin have been cluted from resin, it is oxidated, decolourized, deproteinized and precipitated repeatedly in succession. The titer of refined heparin produced is 156±5u/mg. The proportation is 109.2±3.5 million units/1000kg intestinal mucosa. This technological process is worth spreading and applying.
关键词
猪肠粘膜
连续法
肝素钠
抗凝剂
Swine intestinal mucosa, Unbroken technological process, Refined heparin
