摘要
在已建立了 84K杨叶片外植体再生系统的基础上 ,利用叶盘法首次开展了 84K杨双价耐盐基因 mtl D/gut D的转化研究 ,经诱导不定芽及诱导生根阶段卡那霉素 (选择性抗生素 )连续筛选 ,获得了 1 6株卡那霉素抗性转化植株。抗性植株经 PCR检测 ,有 4株呈阳性。耐盐实验表明 ,3株阳性植株抗 Na Cl能力比对照有不同程度提高。
On the basis of establishment of leaf explant regeneration system of poplar 84K, transformation of mtlD/gutD gene to poplar 84k by means of leaf disc was for the first time undertaken. Through successive selection in the period of shoot and root induction under high lever kanamycin pressure, 16 kanamycin resistant regenerated plants was obtained, of which 4 was positive through PCR analysis. The salt resistant test of these 4 positive plants showed that 3 of them were enhanced in salt tolerance ability to some extent. PCR analysis and salt resistance test preliminarily indicated that the successful transformation had been made.
出处
《西北林学院学报》
CSCD
北大核心
2002年第4期33-37,共5页
Journal of Northwest Forestry University
基金
陕西省科技厅科技攻关项目 (2 0 0 1K0 1-G7)
