摘要
以枯草芽孢杆菌(Bacillus subtilis)Prob1822为研究对象,以热诱激处理(55℃热处理10 min)稳定期菌体存活率为评价指标,通过单因素试验和响应面法研究枯草芽孢杆菌Prob1822复合抗热保护剂的组成。结果表明,单一抗热保护剂以海藻糖、蔗糖及脱脂奶粉抗热保护效果较好;利用响应面法优化复合抗热保护剂配方为海藻糖9.0%、蔗糖5.0%和脱脂奶粉6.8%。在此最优条件下,菌体存活率为(95.24±0.84)%。糖类与蛋白质联用作为复合抗热保护剂比单一保护剂抗热保护效果更好,可减轻喷雾干燥对菌体亚细胞结构及生物大分子损伤,实现枯草芽孢杆菌的抗热保护。
Using Bacillus subtilis Prob1822 as the research object, and the cell survival rate (CSR) in stable stage after thermal-inducible shock at 55 ℃ for 10 min as the evaluation indexes, the formula of the compound anti-thermal protectant of B. subtilis Prob1822 was optimized through single factor tests and response surface methodology. The results indicated that single anti-thermal protectant trehalose, sucrose and skimmed milk powder had better anti-thermal protection effect, and the formula of compound anti-thermal protectant was optimized as trehalose 9.0%, sucrose 5.0% and skimmed milk powder 6.8% using central composite design response surface method. Under the optimal conditions, the cell survival rate was (95.24±0.84)%. The combination of carbohydrate and protein as compound anti-thermal protectant was better than single protectant, which could reduce the subcellular structure and biological macromolecules damage during the spray drying, and provide better protection for B. subtilis.
作者
肖怀秋
李玉珍
林亲录
赵谋明
刘军
周全
姜明姣
XIAO Huaiqiu;LI Yuzhen;LIN Qinlu;ZHAO Mouming;LIU Jun;ZHOU Quan;JIANG Mingjiao(School of Pharmaceutical and Bioengineering,Hunan Chemical Vocational Technology College,Zhuzhou 412000,China;College of Food Science and Technology,Central South University of Forestry and Technology,Changsha 410004,China;School of Food Science and Engineering,South China University of Technology,Guangzhou 510640,China;Hunan Zonwe Pharmaceutical Co.,Ltd.,Zhuzhou 412000,China)
出处
《中国酿造》
CAS
北大核心
2019年第10期49-54,共6页
China Brewing
基金
湖南省自然科学基金科教联合基金项目(2017JJ5020)
关键词
枯草芽孢杆菌
益生菌
响应面法
喷雾干燥
抗热保护剂
Bacillus subtilis
probiotics
response surface methodology
spray drying
anti-thermal protectant
