摘要
为了提高蜡样芽胞杆菌壳聚糖酶活性,对菌株的培养条件进行优化。实验证明在最适温度48℃,最适p H 5. 8,底物浓度1%的条件下,该菌株产酶活力可达6. 034 U/m L。同时证实Mn^2+对壳聚糖酶活力具有明显的促进作用,Al^3+和Cu^2+两种离子对酶活具有抑制作用,利用SDS-PAGE电泳测得该酶相对分子量约44 k Da。结果表明壳聚糖酶具有良好的生物活性,具有进一步工业化的潜力。
In order to increase the chitinase activity of Bacillus cereus,the culture conditions of the strain were optimized. It was found that the enzyme production activity of the strain reached 6. 034 U/m L under the optimum temperature of 48 ℃,optimum p H of 5. 8 and substrate concentration of 1%. At the same time,it was confirmed that Mn^2+ could promote the activity of chitosanase. The two ions of Al^3+and Cu^2+could inhibit the enzyme activity. The relative molecular weight of the enzyme was about 44 kDa by SDS-PAGE. The results indicated that chitosanase had excellent biological activity and had potential for further industrialization.
作者
张颖
郭蔓
张丛丛
陈坤
ZHANG Ying;GUO Man;ZHANG Cong-cong;CHEN Kun(College of Pharmaceutical, Liaocheng University, Shandong Liaocheng 252000;Shandong Ruipu Testing Technology Co., Ltd., Shandong Liaocheng 252000, China)
出处
《广州化工》
CAS
2019年第17期91-93,共3页
GuangZhou Chemical Industry
基金
聊城大学大学生创新创业训练计划项目(No:CXCY2017131),聊城大学“百项工程建设”基金项目
关键词
壳聚糖酶
酶活测定
蜡样芽孢杆菌
chitosanase enzyme
activity determination
Bacillus cereus
