摘要
对核桃青皮多糖(polysaccharide of walnut green husks,JPs)进行分离纯化,并对得到的多糖进行单糖组成及体外抗氧化活性分析。利用超声波辅助提取JPs,经醇沉、除蛋白、透析等一系列处理后,先后利用DEAE-Sepharose和Sephadex G-100柱色谱分离纯化JPs得到了两种均一多糖(JPs-1-1和JPs-2-1);利用高效凝胶渗透色谱法测定两种均一多糖的分子质量分别为5.45×10^4、5.22×10^4 u;利用PMP-柱前衍生高效液相色谱法测定了两种均一多糖的单糖组成,均是由鼠李糖(Rha)、葡萄糖醛酸(GlcA)、半乳糖(Gal)、葡萄糖(Glc)、半乳糖醛酸(GalA)、木糖(Xyl)及阿拉伯糖(Ara)以不同的物质的量比组成。总抗氧化能力实验、1,1-二苯基-2-苦味基肼自由基和羟自由基清除实验结果表明JPs及分离纯化得到的两种均一多糖均具有较好的抗氧化活性,而且抗氧化活性的能力与样品的质量浓度呈正相关。
In this study,polysaccharides named as JPs were ultrasonically extracted from walnut green husks.After sequential treatments of ethanol precipitation,deproteinization and dialysis,JPs were fractionated by DEAE-Sepharose and Sephadex G-100 column chromatography into two homogenous fractions(JPs-1-1 and JPs-2-1).The molecular masses of JPs-1-1 and JPs-2-1 were measured to be 5.45×10^4 and 5.22×10^4 u by high performance gel filtration chromatography,respectively.Monosaccharide analysis by precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone(PMP)coupled to high performance liquid chromatography(HPLC)indicated that both polysaccharides were composed of rhamnose,glucuronic acid,galactose,glucose,galacturonic acid,xylose and arabinose,but in different molecular ratios.JPs and its fractions possessed a good antioxidant activity in terms of ferric-reducing antioxidant power and scavenging activities against hydroxyl and 1,1-diphenyl-2-picrylhydrazyl(DPPH)radicals in a dose-dependent manner within a certain concentration range.
作者
谢东雪
陆娟
王月
王超雪
陈瑞战
高佳欣
XIE Dongxue;LU Juan;WANG Yue;WANG Chaoxue;CHEN Ruizhan;GAO Jiaxin(College of Chemistry, Changchun Normal University, Changchun 130032, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第18期281-286,共6页
Food Science
基金
长春师范大学研究生教育创新基金项目（cscxy2018035）
关键词
核桃青皮
多糖
纯化
抗氧化活性
green walnut husks
polysaccharides
purification
antioxidant activity
