摘要
目的建立UPLC法同时测定玉屏风颗粒(黄芪、白术、防风)中升麻素苷、毛蕊异黄酮-7-O-β-D-葡萄糖苷、升麻素、5-O-甲基维斯阿米醇苷、芒柄花苷、毛蕊异黄酮、亥茅酚苷、芒柄花素、白术内酯Ⅲ的含有量。方法该药物75%甲醇提取液的分析采用ACQUITY UPLC? BEH C18色谱柱(2.1 mm×150 mm,1.8μm);流动相乙腈-水,梯度洗脱;体积流量0.20 mL/min;柱温30℃;检测波长220 nm。结果 9种成分在各自范围内线性关系良好(r>0.999 0),平均加样回收率96.40%~106.82%,RSD 2.14%~3.85%。结论该方法简便、快速、准确,可用于玉屏风颗粒的质量控制。
AIM To establish a UPLC method for the simultaneous content determination of prim-O-glucosylci-mifugin,calycosin-7-O-β-D-glucoside,cimifugin,4'-O-β-glucopyranosyl-5-O-methylvisamminol,ononin,calycosin,sec-O-glucosylhamaudol,formononetin,atractylenolideⅢin Yupingfeng Granules(Astragali Radix,Atractylodis macrocephalae Rhizoma,Saposhnikoviae Radix).METHODS The analysis of 75%methanol extract of this drug was performed on a 30℃thermostatic ACQUITY UPLC■BEH C18 column(2.1 mm×150 mm,1.8μm),with the mobile phase comprising of acetonitrile-water flowing at 0.20 mL/min in a gradient elution manner,and the detection wavelength was set at 220 nm.RESULTS Nine constituents showed good linear rela-tionships within their own ranges(r>0.999 0),whose average recoveries were 96.40%-106.82%with the RSDs of 2.14%-3.85%.CONCLUSION This simple and accurate method can be used for the rapid quality control of Yupingfeng Granules.
作者
廖小芳
毕晓黎
胥爱丽
袁春平
李养学
高永坚
LIAO Xiao-fang;BI Xiao-li;XU Ai-li;YUAN Chun-ping;LI Yang-xue;GAO Yong-jian(The Fifth Clinical Medicine College,Guangzhou University of Chinese Medicine,Guangzhou 510405,China;Guangdong Provincial Engineering and Technology Research Institute for Traditional Chinese Medicine,Guangzhou 510095,China;Guangdong Provincial Key Laboratory for Research and Development of Traditional Chinese Medicine,Guangzhou 510095,China;Sinopharm Group Guangdong Medi-world Pharmaceutical Co.,Ltd.,Foshan 528303,China)
出处
《中成药》
CAS
CSCD
北大核心
2019年第8期1778-1781,共4页
Chinese Traditional Patent Medicine
基金
广东省名优中成药二次开发项目(粤中医办函[2017]19)
广东省科技计划项目(2017A070701017)
关键词
玉屏风颗粒
化学成分
UPLC
Yupingfeng Granules
chemical constituents
UPLC
