摘要
目的探讨高剂量D-半乳糖复制C57BL/6J衰老小鼠模型的效果。方法选用30只9周龄雄性C57BL/6J小鼠,随机分为3组:500mg/(kg·d)D-半乳糖组、1000mg/(kg·d)D-半乳糖组及对照组,持续皮下注射8周后解剖小鼠,测定血液和组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHPx)及丙二醛(MDA)等,Westernblotting检测组织中血红素加氧酶-1(HO-1)的表达,并对部分组织行苏木精-伊红染色。结果1000mg/(kg·d)D-半乳糖组小鼠出现不同程度的脱毛现象。D-半乳糖组小鼠的SOD和GSH-Px低于对照组(P<0.05),而MDA和HO-1高于对照组(P<0.05)。D-半乳糖组小鼠肝、脑组织有不同程度的衰老相关病理学改变。结论D-半乳糖可以成功诱导小鼠衰老,是复制小鼠衰老模型的较好选择。
Objective To evaluate the effect of the high-dose D-galactose inducing aging model in C57BL/6J mice. Methods Thirty C57BL/6J mice were divided into 3 groups: 500 mg/(kg·d) D-galactose group, 1000 mg/(kg·d) D-galactose group and the 0.9% saline group by daily subcutaneous injection for 8 weeks. The superoxide dismutase, glutathione peroxidase and malondialdehyde levels in serum, liver and brain were evaluated. The expression of heme oxygenase-1 expression in liver and brain was detected by western blotting. Tissues were observed by H&E staining. Results There were no differences in the appearance between 500 mg/(kg·d) group and control group, but the mice in 1000 mg/(kg·d) group showed the phenomenon of hair loss. The activities of superoxide dismutase and glutathione peroxidase significant decreased in the groups treated with D-galactose compared with control group (P < 0.05), and the concentration of the malondialdehyde was significantly higher than the control group (P < 0.05). The expression of heme oxygenase-1 was increased in the D-galactose group. There were Pathological changes in D-galactose groups. Conclusions The D-galactose could induce the aging model and the dose of 1000 mg/(kg·d) D-gal could cause the appearance change in C57BL/6J mice.
作者
赵焕东
胥洪鹃
李坚
任彩萍
陈玉祥
Huan-dong Zhao;Hong-juan Xu;Jian Li;Cai-ping Ren;Yu xiang Chen(Operation Room, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China;Cancer Research Institute, School of Basic Medical Science, Central South University, Changsha, Hunan 410078, China;Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China;Xiangya School of Pharmaceutical Sciences, Central South University,Changsha, Hunan 410013, China)
出处
《中国现代医学杂志》
CAS
2019年第16期10-16,共7页
China Journal of Modern Medicine
