摘要
目的:观察艾灸对胃热证大鼠胃黏膜组织形态的影响。方法:70只Wistar大鼠按随机数字表法随机分为7组,分别为:正常组、胃热证组、胃热艾灸组、胃热艾灸对照组、胃寒证组、胃寒艾灸组、胃寒艾灸对照组。除正常组外,胃热组用辣椒素和乙醇溶液灌胃,胃寒组用冰水灌胃及凉水泡浴等分别复制胃热证、胃寒证模型。造模后除正常组、胃热证组、胃寒证外,其余分别予艾灸干预12天。结果:与正常组比较,胃热证组大鼠躁动、兴奋,胃寒证组精神不振、踡缩、懒动等,胃热证组和胃寒证组的胃黏膜损伤指数明显升高(P<0.05),胃黏膜组织病理学观察可见黏膜坏死脱落,溃疡形成,间质出现水肿,细胞间隙增宽明显,炎细胞浸润广泛;与模型组比较,胃热艾灸组可以改善大鼠躁动、兴奋等胃热证的证候,胃寒艾灸组可以改善大鼠精神不振、踡缩、懒动等胃寒证的证候,胃热艾灸组和胃寒艾灸组的胃黏膜损伤指数明显下降(P<0.05),胃热艾灸组和胃寒艾灸组的胃黏膜组织病理学观察可见胃黏膜浅层有细胞脱落,无明显溃疡形成;胃热艾灸对照组和胃寒艾灸对照组无论在一般行为学的证候方面,还是在胃黏膜损伤指数和组织病理学方面均未见明显变化;胃热证组和胃寒证组大鼠的胃黏膜细胞中NT和Survivin的表达下降,艾灸胃经穴可以上调胃黏膜细胞中NT和Survivin的水平,而艾灸对照组则未见明显变化。结论:艾灸可以促进胃热证大鼠胃黏膜损伤修复,在一定程度上证明“热证可灸”具有可行性。
Objective: To observe the effect of moxibustion on gastric mucosal histomorphology in rats with Stomach-heat syndrome. Methods: 70 Wistar rats were randomly divided into seven groups: the normal group, the Stomach-heat syndrome group, the Stomach-heat moxibustion group, the Stomach-heat moxibustion control group, the Stomach-cold syndrome group, the Stomach-cold moxibustion group and the Stomach-cold moxibustion control group. The Stomach-heat model was set up by gastric gavage with capsaicin and ethanol solution;whereas, the Stomach-cold model was established by gastric gavage with cold water and by cold bath. After modeling, the rats were intervened by moxibustion for 12 days in the moxibustion groups. Results: Compared to the normal control, the rats in the model group of Stomach-heat syndrome were restless and excited, but the rats in the model group of Stomach-cold syndrome were depressed, curled up and lazy. The injury index of gastric mucosa in these two model groups increased significantly( P <0.05). Histopathological observation of gastric mucosa showed necrosis and peeling of mucosa, ulcer formation, interstitial edema, widening of intercellular space and extensive infiltration of inflammatory cells. Compared to the corrsponding model group, the symptoms of restlessness and excitement were relieved in the Stomach-heat moxibustion group, and the symptoms of listlessness, curling up and laziness were reduced in the Stomach- cold moxibustion group. The gastric mucosal injury indexes were also decreased significantly in the two moxibustion groups( P < 0.05). Histopathological observation of gastric mucosa showed that there were cell exfoliation in superficial layer of gastric mucosa without obvious ulcer formation. However, in the two moxibustion control groups, there were no significant changes in general behavioral symptoms, injury index of gastric mucosa and histopathology. The expressions of NT and Survivin were decreased in gastric mucosa cells in the rats with the Stomach- heat syndrome and Stomach-cold syndrome. Moxibustion applied to Stomach meridian could up-regulate the levels of NT and Survivin in gastric mucosa cells, whereas moxibustion control groups showed no significant changes. Conclusion: Moxibustion can promote the repair of gastric mucosa injury in rats with Stomach- heat syndrome, which proves that moxibustion applied to heat syndrome is feasible.
作者
谢宇锋
余云进
杨锦兰
陈赟
冯军
杨宗保
XIE Yufeng;YU Yunjin;YANG Jinlan;CHEN Yun;FENG Jun;YANG Zongbao(Shenzhen Hospital of Guangzhou University of Chinese Medicine,Shenzhen 518000,China;Guangzhou Universityof Chinese Medicine,Guangzhou 510000,China;Medical College of Xiamen University,Xiamen 361005,China)
出处
《针灸临床杂志》
2019年第6期68-73,共6页
Journal of Clinical Acupuncture and Moxibustion
基金
广东省自然科学基金,编号:2017A030313748
广东省中医药局中医药科研项目,编号:20191294
深圳市卫生计生委科研项目,编号:201607061
深圳市福田区卫生公益性科研项目,编号:FTWS20160033
关键词
艾灸
胃热证
胃黏膜修复
热证可灸
组织形态学
Moxibustion
Stomach-heat syndrome
Gastric mucosal repair
Moxibustionapplied to heat syndrome
Histomorphology
