摘要
目的:探讨水浸应激型胃溃疡寒、热证模型的形成机制及寒、热方剂对该模型的作用机理及其方证相应的客观规律。方法:采用寒、热因素结合水浸应激法建立大鼠胃溃疡寒、热证模型,采用酶联免疫法检测受试大鼠血栓素B2(TXB2)和神经降压素(NT)的含量。结果:各模型组与空白组相比,TXB2含量均有不同程度的升高,且差异显著(P<0.01)。且水浸应激型胃溃疡寒、热证之病证结合模型较单证、单病模型TXB2含量明显升高。各模型组与空白组比较NT含量均明显降低,有显著性差异(P<0.05或P<0.01)。但各模型组间比较无明显差异。大黄黄连泻心汤、理中丸可分别显著降低热模型组、寒模型组TXB2含量,升高NT含量(P<0.05或P<0.01)。结论:通过"方证相应"理论验证了"病证结合"胃溃疡寒、热证模型的可行性,大黄黄连泻心汤对热性胃溃疡、理中丸对寒性胃溃疡的治疗作用,可通过降低血清TXB2、升高血清NT的含量而实现。
Objective：To explore the forming mechanism of gastric ulcer model induced by bondage water immersion stress combined with cold or heat syndrome.And to observe the basic mechanism of action and objective regularity of ＂formula-syndrome corresponding＂ of cold or heat formula on gastric ulcer model combined with cold or heat syndrome.Methods： Adopting cold or heat factors combined with bondage water immersion stress methods to establish gastric ulcer model combined with cold and heat syndrome.Using euzymelinked immunosorbent assay to detect the contents of TXB_2,NT in serum.Results：Compared with the blank group,the contents of TXB_2 in serum increased remarkably in each model groups（P0.01）.The contents of TXB_2 increased more remarkably in ＂disease-syndrome combination＂ model than those in simple syndrome or simple disease model.Compared with the blank group,the contents of NT in serum decreased remarkably in each model group（P0.05 or P0.01）.But there is no obvious difference among the model groups.Dahuang Huanglian Xiexin decoction or Lizhong pill could respectively decreased the contents of TXB_2 and increase the contents of NT in serum of heat-ulcer group or cold-ulcer group obviously（P0.05 or P0.01）.Conclusions：On the basis of the theory of ＂formula-syndrome corresponding＂,verifying the feasibility of duplicating ＂disease-syndrome combination＂ model,that is gastric ulcer model combined with cold or heat syndrome.The therapeutic action of Dahuang Huanglian Xiexin decoction on heat-gastric ulcer,or Lizhong pill on cold-gastric ulcer can both be achieved by decreasing the contents of TXB_2,increasing the contents of NT in serum.
出处
《中医药学报》
CAS
2011年第6期21-23,共3页
Acta Chinese Medicine and Pharmacology
基金
"十一五"国家科技支撑计划项目-"方证相应"模式之复方药理研究(2006BAI08B04-03)
关键词
束缚水浸应激
胃溃疡
寒、热证
TXB2
NT
Bondage water immersion stress
Gastric ulcer
Cold or heat syndrome
TXB_2
NT
