摘要
目的 研究红景天苷对脑缺血/再灌注大鼠补体成分的作用,进一步探讨红景天苷神经保护的作用机制。方法♂成年SD大鼠45只,随机分为假手术(Sham)组、模型(MCAO)组、红景天苷(MCAO+Sal)组,线栓法制备大鼠大脑中动脉闭塞模型(MCAO),红景天苷浓度为50 mg·kg^-1 ·d^-1 ,连续给药6 d。检测脑组织线粒体内Bax、Bcl-xl蛋白表达,基因芯片聚类分析,qPCR检测补体成分C1qa、C1qb、 C1qc等 mRNA表达,Western blot检测补体C3的表达,免疫荧光法检测C3和NeuN的表达。结果与MCAO组相比,红景天苷给药后,降低线粒体内Bax蛋白表达,促进Bcl-xl蛋白表达;经基因芯片聚类分析及qPCR检测,红景天苷能够抑制C1qa、C1qb、C1qc等 mRNA补体相关基因表达;经Western blot及免疫荧光法检测,红景天苷能够抑制C3的表达,同时促进NeuN的表达。结论红景天苷能够抑制补体成分,尤其是补体中枢成分C3,对MCAO大鼠具有神经保护作用。
Aim To study the effects of salidroside on the complement components in MCAO rats and to further explore the mechanism of neuroprotection of salidroside.Methods Forty-five healthy male Sprague-Dawley rats were randomly divided into sham operation(Sham) group, model(MCAO) group, and salidroside(MCAO+Sal) group.Rats were administered salidroside, or vehicle, daily for 6 days, after middle cerebral artery occlusion(MCAO)2 h and reperfusion1 h.The protein expressions of C3 and brain tissue mitochondrial Bax, Bcl-xl were detected by Western blot.The mRNA expressions of C1qa, C1qb, C1qc, C2, C3, C3a, C4a, C5a, Cfh, Cfd were detected by qPCR.Gene chip technology was used for detection.The expressions of C3 and NeuN were detected by immunofluorescence.Results Compared with MCAO group, salidroside could reduce the protein expression of Bax protein in mitochondria and promote the expression of Bcl-xL protein after salidroside treatment.By gene chip cluster analysis and qPCR detection, salidroside could inhibit the expression of complement-related genes such as C1qa, C1qb, C1qc, C2, C3, C3a, C4a, C5ar, Cfh and Cfd mRNA.According to Western blot and immunofluorescence, salidroside inhibited the expression of C3 and promoted the expression of NeuN.Conclusions Salidroside can inhibit the complement component, especially the action of the complement central component C3, exerting neuroprotective effects on MCAO rats.
作者
黄鑫
林昱
杨泽霖
张小琴
谢秀利
汪旭雯
唐宇恒
刘俊杰
赖文芳
洪桂祝
HUANG Xin;LIN Yu;YANG Ze-lin;ZHANG Xiao-qin;XIE Xiu-li;WANG Xu-wen;TANG Yu-heng;LIU Jun-jie;LAI Wen-fang;HONG Gui-zhu(College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122,China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2019年第7期967-972,共6页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81603323,81473382)
福建中医药大学资助项目(No X2018002-协同)
2018年福建省教育厅杰青项目
关键词
红景天苷
脑缺血/再灌注
补体成分
神经保护
C3
凋亡
salidroside
cerebral ischemia/reperfusion
complements
neuroprotection
C3
apoptosis
