摘要
为促进天麻的驯化栽培及合理采收,以贵州乌天麻为试验材料,采用高效液相色谱-紫外检测(HPLCUV)方法,研究了乌天麻中天麻素(Gastrodin)、对羟基苯甲醇(P-Hydroxybenzylalcohol)、对羟基苯甲醛(PHydroxybenzaldehyde)的含量,并对不同采收期贵州乌天麻中3种成分的动态变化规律进行分析。结果表明:天麻素、对羟基苯甲醇、对羟基苯甲醛的线性范围分别为0.010 16~1.016 00,0.105 2~2.104 0,0.010 21~0.204 20μg(r≥0.9997),检测限分别为0.67,0.35,0.34ng。定量限分别为2.03,1.05,1.02ng。精密度、稳定性(24h)、重复性试验的RSD%均小于2%(n=6);加标回收率为99.94%~100.54%,RSD为0.98%~1.64%(n=9)。在不同的采收期,贵州乌天麻中3种成分含量有差异,其中天麻素在1月采收时含量较高,对羟基苯甲醇在11月采收含量较高,而对羟基苯甲醛在不同采收期含量变化不大;但不同采收期乌天麻中天麻素与对羟基苯甲醇含量之和均大于0.25%,符合药典规定。所建立的HPLC-UV方法,操作简单,准确度高,可用于贵州乌天麻的质量控制。
In order to promote domestication cultivation and rational harvest of Gastrodiae rhizoma ,an HPLC-UV method was established for simultaneous determination of gastrodin,p-Hydroxybenzylalcohol and p-Hydroxybenzaldehyde in Gastrodiae rhizoma harvested on different periods.The detection wavelength was set at 225 nm and the column was maintained at 30 ℃.10 μL solution was injected for analysis.The results showed that the linear ranges for gastrodin,P-Hydroxybenzylalcohol and P-Hydroxybenzaldehyde were 0.010 16- 1.016 00, 0.105 2- 2.104 0 and 0.010 21~0.204 20 μg.The limits of detection were 0.67,0.35,0.34 ng,and the limits of quantification were 2.03,1.05,1.02 ng,respectively.The relative standard deviations(RSDs) of precision,stability(24 h),and reproducibility tests for all the three components were lower than 2.0%(n=6).The recovery test for accuracy was conducted and the results were 99.94%-100.54%(RSD=0.98%-1.64%, n=9 ).The results INDICATed that gastrodin was highest in sample of harvest period in January,while the content of P-Hydroxybenzylalcohol was highest in that of November.The content of P-Hydroxybenzaldehyde was maintained in low level of all harvest periods.However,the sum contents of gastrodin and P-Hydroxybenzylalcohol in all the sample of different harvest periods were higher than 0.25%,which meet the requirement of China Pharmacopoeia.The established HPLC method was simple,reliable and suitable for the quality control of G.rhizoma .
作者
邓青芳
马风伟
费强
肖娟
曹森
杨碧仙
王瑞
DENG Qing-fang;MA Feng-wei;FEI Qiang;XIAO Juan;CAO Sen;YANG Bi-xian;WANG Rui(Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment of Guizhou Province,Guizhou Normal University,Guiyang 550001,China;Food and Pharmaceutical Engineering Institute,Guiyang University,Guiyang 550005,China;Shenqi Ethnic Medicine College of Guizhou Medical University,Guiyang 550005,China)
出处
《黑龙江农业科学》
2019年第6期113-118,共6页
Heilongjiang Agricultural Sciences
基金
贵州省教育厅青年科技人才成长项目(黔教合KY字[2017]123,[2016]249和[2018]291)
贵阳学院博士科研启动经费项目(GYU-ZRD[2018]-022)
贵阳市科技局贵阳学院专项资金(GYU-KYZ[2018]01-01)
贵州省高等学校大学生创新创业训练项目(2018520804,2018520805)
贵阳学院教学质量与教学改革项目(2017028)
贵阳学院实验室开放经费项目(2018027510706)
关键词
天麻
HPLC法
含量测定
采收期
Gastrodiae rhizoma
HPLC method
content determination
harvest period
dynamic analysis
