摘要
[Objectives]This study was conducted to determine the content of total flavonoids in ginkgo ( Ginkgo biloba L.) leaves.[Methods]The content of total flavonoids from ginkgo leaves was determined by reversed phase-high performance liquid chromatography (RP-HPLC).The flavonoid glycosides were first extracted with methanol,and hydrolyzed with hydrochloric acid solution to prepare a test solution.Platisil ODS C18 column (150 mm×4.6 mm,5 μm) and the mobile phase V methanol ∶ V water (0.4% phosphoric acid solution)=85∶ 15 were selected for HPLC separation.The HPLC separation was performed with the column at a column temperature of 25℃ using the mobile phase at a flow rate of 1 ml/min.The sample size was 10 μl,and detection was performed with an Agilent HPLC ultraviolet detector at 360 nm.[Results]The reference substance,quercetin,had good linearity in the range of 0.002 6-0.052 0 g/L,with a correlation coefficient of 0.999 7;and the RSD was 1.26%.[Conclusions]The determination method has rapid and simple operation with accurate results and is good in repeatability.This method is suitable for the determination of content of total flavonoids in ginkgo leaves.
[Objectives] This study was conducted to determine the content of total flavonoids in ginkgo（Ginkgo biloba L.） leaves. [Methods] The content of total flavonoids from ginkgo leaves was determined by reversed phase-high performance liquid chromatography（RP-HPLC）. The flavonoid glycosides were first extracted with methanol, and hydrolyzed with hydrochloric acid solution to prepare a test solution. Platisil ODS C18 column（150 mm×4.6 mm, 5 μm） and the mobile phase Vmethanol∶Vwater（0.4% phosphoric acid solution）=85∶15 were selected for HPLC separation. The HPLC separation was performed with the column at a column temperature of 25℃ using the mobile phase at a flow rate of 1 ml/min. The sample size was 10 μl, and detection was performed with an Agilent HPLC ultraviolet detector at 360 nm. [Results] The reference substance, quercetin, had good linearity in the range of 0.0026-0.0520 g/L, with a correlation coefficient of 0.9997; and the RSD was 1.26%. [Conclusions] The determination method has rapid and simple operation with accurate results and is good in repeatability. This method is suitable for the determination of content of total flavonoids in ginkgo leaves.
基金
Supported by Guilin Science and Technology Bureau Project(20100305)
Guangxi "2011 Collaborative Innovation Center"-Zhuang Yao Medicine Collaborative Innovation Center Project(G2013[20])
