摘要
银杏叶经水提、醇沉等得到银杏叶多糖（ＬＧＢＰ）。将ＬＧＢＰＨ Ｓｅｐｈａｄｅｘ Ｇ１００层析柱，分离得到ＬＧＢＰ－Ａ和ＬＧＢＰ－Ｂ两种多糖，经电泳与凝胶柱柱层析鉴定均为单一多糖。平均分子量分别为１１×１０＾４和２×１０＾４。经纸层析与高效液相色谱证实其单糖组成分别是：ＬＧＢＰ－Ａ：Ｄ－葡萄糖，Ｌ－鼠李糖、Ｄ－木糖；ＬＧＢＰ－Ｂ：Ｄ－葡萄糖。
The Polysaccharide from the leave of Ginkgu bitoba L (LGBP) was obtained by extracting and precipitating. LGBP-A and LGBP-B were isolated by Sephadex G100 gel filtration, which were determined to be chemically homogeneous by means of electrophoresis and gel column chromatogra-phy. The average MW was found separately to be 110 000 and 20 000. The chemical composition was separately LGBP-A; D-glucose, L-rhamnose,D-xylose; LGBP-B: D-glucose by means of high performance liquid chromatogrophy and paper chromatogrophy.
出处
《中国生化药物杂志》
CAS
CSCD
1996年第4期157-159,共3页
Chinese Journal of Biochemical Pharmaceutics
关键词
银杏叶
多糖
分离纯化
鉴定
Leave of Ginkgo biloba L, Polysaccharide, Isolation and purification, Determination
