摘要
通过对香蕉Aux/IAA (auxin/indole-3-acetic acid)基因家族密码子使用偏好性进行研究为进一步的功能研究尤其是异源表达验证提供参考。本研究采用CodonW、EMBOSS在线程序、SPSS和Origin软件分析了香蕉Aux/IAA基因家族的密码子使用偏好性参数,并和香蕉基因组以及几种遗传转化再生体系成熟的模式植物基因组做了比较。结果表明:家族基因密码子偏好性较弱,偏好使用G或C,且以G或C结尾,与单子叶植物密码子偏好特性较为一致,UUG、GCA、GAC、GGA是该家族的最优密码子,具有偏好性的密码子和最优密码子与香蕉基因组差异较大。香蕉Aux/IAA基因家族密码子偏好性主要受突变压力影响。在密码子改造和优化的基础上,大肠杆菌原核表达系统适合作为该家族成员的异源表达系统,烟草可作为该家族基因遗传转化的受体。本研究结果有助于开展香蕉Aux/IAA基因家族的功能研究。
Research on the codon usage bias of the banana Aux/IAA(auxin/indole-3-acetic acid) gene family could provide reference for further functional studies, especially for verification of heterologous expression. In this study,CodonW, EMBOSS online programs, SPSS and Origin software were used to analyze the codon usage bias of the banana Aux/IAA gene family, and compared with the banana genome and several mature model plant genomes of the genetic transformation and regeneration system. The results showed that the genetic codon bias of the family was weak, preferring to use G or C, and ending with G or C, which was consistent with monocotyledon codon preference characteristics. UUG, GCA, GAC, GGA were the family’s optimal codons. Preferred codons and optimal codons differed greatly from the banana genome. Codon bias of banana Aux/IAA gene family was mainly affected by mutation pressure. On the basis of codon modification and optimization, the E. coli prokaryotic expression system was suitable as a heterologous expression system for the family members, and tobacco could be used as a genetic transformation receptor for the family. This study could lay a foundation for the study of the function of banana Aux/IAA gene family.
作者
孙雪丽
郝向阳
李蓉
项蕾蕾
彭丽云
刘范
田娜
程春振
赖钟雄
王天池
Sun Xueli;Hao Xiangyang;Li Rong;Xiang Leilei;Peng Liyun;Liu Fan;Tian Na;Cheng Chunzhen;Lai Zhongxiong;Wang Tianchi(Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, 350002)
出处
《分子植物育种》
CAS
CSCD
北大核心
2019年第8期2467-2475,共9页
Molecular Plant Breeding
基金
国家自然科学基金项目(31601713)
福建省中青年教师教育科研项目(JAT160166)
福建农林大学"校杰出青年科研人才"计划项目(xjq201721)
福建农林大学博士后启动基金(132300197)共同资助
国家现代农业产业技术体系建设专项资金项目(CARS-32-11)
