摘要
近年来,肉类掺假事件时有发生,给消费者和相关食品安全监督部门造成一定的困扰,同时威胁着消费者的身体健康。传统意义上的检测方法如酶联检测法及PCR法均存在一定的局限性,限制其在市场上进行快速的肉类鉴别。环介导等温扩增(LAMP)是一种新型核酸扩增技术,其灵敏度高、特异性强,不依赖专业仪器设备,操作简单,已开始被广泛应用于食源性微生物的检验。本文从LAMP原理出发,阐述了LAMP技术在肉源性检测中的应用,对LAMP技术的发展前景进行了展望。
In recent years, meat adulteration incidents have occurred from time to time, causing a certain amount of trouble to consumers and related food safety supervision departments, while threatening the health of consumers. Traditional detection methods such as ELISA and PCR have some limitations, which restrict the rapid identification of meat in the market. Application and prospect of loop-mediated isothermal amplification(LAMP) is a novel nucleic acid amplification technique with high sensitivity, specificity, no dependence on professional instruments and simple operation. It has been widely used in the detection of food-borne microorganisms. Based on the principle of LAMP, this paper expounds the application of LAMP technology in meat-borne detection, and prospects the development prospect of LAMP technology.
作者
娄宇飞
魏昌盛
胡瑞芳
王洪宝
昝林森
LOU Yu-fei;WEI Chang-sheng;HU Rui-fang;WANG Hong-bao;ZAN Lin-sen(College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100)
出处
《中国牛业科学》
2019年第1期33-38,44,共7页
China Cattle Science
基金
国家肉牛牦牛产业技术体系项目(CARS-37)
关键词
环介导等温扩增(LAMP)
肉源检测
应用
展望
loop-mediated isothermal amplification(LAMP)
meat source detection
application
prospect.
