摘要
畜禽食品安全一直是全世界公共健康的关注焦点。环介导等温扩增(LAMP)是在等温条件下对目标DNA序列进行扩增的一种技术,在微生物检测方面,与常规技术相比,具有方法简单、特异性强、便于观察结果、不需要精密仪器等优势。该文主要对LAMP在畜禽微生物检测上的应用及进展进行综述,并对其优缺点进行分析。
Safety of livestock and poultry foods is always the focus of public health in the world.Loop mediated isothermal amplification( LAMP) is a technique for amplification of target DNA sequences under isothermal conditions.In microorganism detection,LAMP has the advantages of simplicity,strong specificity,easy observation and no need of precision instruments compared with conventional technology.In this paper,the application and progress of LAMP in microorganism detection of livestock and poultry were reviewed,and its advantages and disadvantages were analyzed.
作者
李璐璐
张庆
骆延波
张印
胡明
赵效南
齐静
刘玉庆
Li Lulu;Zhang Qing;Luo Yanbo;Zhang Yin;Hu Ming;Zhao Xiaonan;Qi Jing;Liu Yuqing(Institute of Animal Husbandry and Veterinary Medicine,Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Animal Breeding and Disease Controlling,Jinan 250100,China)
出处
《山东农业科学》
2020年第7期166-172,共7页
Shandong Agricultural Sciences
基金
国家重点研发专项计划项目(2016YFD0501301)
国家科技重大专项(2018ZX10733402-013)
传染病预防控制国家重点实验室开放课题项目(2019SKLID317)
山东省现代农业产业技术体系家禽创新团队环境控制岗位专家项目(SDAIT-13-011-09)
山东省农业科学院农业科技创新工程项目(CXGC2018E10,CXGC2017A01)。
关键词
畜禽
微生物检测
环介导等温扩增
Livestock and poultry
Microorganism detection
Loop mediated isothermal amplification(LAMP)
