摘要
目的探讨臭氧水灌洗阴道对正常阴道微生态及阴道乳杆菌的影响。方法选取2016年4月至10月于北京清华长庚医院女性职工中招募阴道微生态检查结果正常的志愿者30例,非月经期每天接受臭氧水灌洗阴道5min,连续3 d。分别在初次阴道臭氧水灌洗前、末次灌洗后的第1周、第2周、第3周留取阴道分泌物,进行阴道微生态检测及阴道微生物16S rRNA高通量测序后对菌群多样性和丰度进行分析。结果微生态检查结果正常的女性接受臭氧水灌洗阴道前后,其阴道微生态、阴道内菌群多样性及乳杆菌无显著差异(cor.p绝对值<0.2)。结论臭氧水灌洗阴道不破坏正常阴道微生态环境,对乳杆菌无显著影响。
Objective To explore the changes of normal vaginal micro-ecological environment after vaginal lavage with ozonated water and its effect on lactobacillus.Methods From April to October 2016,30 volunteers with normal vaginal microecological examination results were recruited from female workers in Beijing Tsinghua Changgung Hospital and selected to receive vaginal lavage with ozonated water,during non-menstrual periods 5 minutes per day,for 3 consecutive days.The vaginal secretions were taken before the first vaginal ozone lavage,the first week,the second week and the third week after the last lavage,and the microecological examination and the difference in the diversity and abundance of the vaginal microbial community were analyzed after 16 S rRNA high-through put sequencing.Results Before and after healthy women received ozone water for vaginal lavage,there were no significant differences in the microflora evaluation of vaginal secretions,vaginal microbial community diversity or lactobacillus(|cor.p|<0.2).Conclusion Ozonated water for vaginal lavage does not destroy the normal vaginal microecology and has no significant effect on lactobacilli.
作者
张琼琼
刘瑛
张蕾
陈锐
陶址
黄振宇
吕涛
廖秦平
ZHANG Qiong-qiong;LIU Ying;ZHANG Lei;CHEN Rui;TAO Zhi;HUANG Zhen-yu;LYU Tao;LIAO Qin-ping(Department of Obstetrics and Gynecology,Beijing Tsinghua Changgung Hospital,School of Clinical Medicine,TsinghuaUniversity,Beijing 102218,China)
出处
《中国实用妇科与产科杂志》
CAS
CSCD
北大核心
2019年第4期450-454,共5页
Chinese Journal of Practical Gynecology and Obstetrics
基金
国家自然科学基金(81671409)
北京市医院管理局临床医学发展专项经费(XMLX201605)
关键词
臭氧水
阴道灌洗
阴道微生态
乳杆菌
高通量测序
ozonated water
vaginal lavage
vaginal microecology
lactobacillus
high-throughput sequencing
