摘要
从某焦化厂污水处理系统的活性污泥中筛选到一株喹啉高效降解菌WUST-qu,对其形态、分类地位、降解特征和生长动力学进行表征。结果表明,该菌株为革兰氏阴性菌,有芽孢,长为1.15μm±0.08μm(n=20),宽为0.26μm±0.01μm (n=20);16S rRNA基因核苷酸序列比对结果表明,该菌株属产碱杆菌属微生物(Alcaligenes sp)。菌株WUST-qu能在初始pH为5.0～9.0内有效降解喹啉,最适初始pH为中性到略偏碱性(7.0～8.0)。在该条件下,菌株能在接种后的48 h内将最高初始质量浓度为700 mg·L^(-1)的喹啉完全降解。进一步研究表明,菌株WUST-qu不仅能够有效降解喹啉,还能有效降解苯酚,在MSM初始pH为8.0的条件下,菌株WUST-qu能在32 h内将初始质量浓度为700 mg·L^(-1)的苯酚完全降解。其以喹啉为唯一碳源生长时的动力学参数为μ_(max)=1.657 6 h^(-1),K_s=36.42 mg·L^(-1),K_i=81.418 mg·L^(-1)。
In this study,a potent quinoline-degrading bacterial strain WUST-qu was isolated from the activated sludge of the wastewater treatment system of a coke plant,and the morphological properties,taxonomic status,degrading characteristics,and kinetics of growth were characterized.The results demonstrated that the strain WUST-qu is Gram negative and sporulating with a length of 1.15μm±0.08μm(n=20),and a width of 0.26μm±0.01μm(n=20).Partial 16S rDNA sequence alignment indicated that WUST-qu belongs to the genus of Alcaligenes.The strain could effectively degrade quinoline at between pH 5.0 and 9.0,but the ideal pH to degrade quinoline is neutral or slightly basic(7.0-8.0),under which the strain could completely degrade quinoline at a maximal initial concentration of 700 mg·L^-1 within 48 h upon inoculation.The further study indicated that the strain WUST-qu also degrades phenol effectively.The strain could completely degrade phenol at 700 mg·L^-1 within 32 h at an initial pH of 8.0.The kinetic parameters for growth with quinoine as the sole carbon were as follow:μmax=1.657 6 h-1,K s=36.42 mg·L^-1,K i=81.418 mg·L^-1.
作者
刘建忠
易红磊
翟赟
黄皓
陈俊
周卫
秦晓蓉
LIU Jianzhong;YI Honglei;ZHAI Yun;HUANG Hao;CHEN Jun;ZHOU Wei;QIN Xiaorong(School of Chemistry and Chemical Engineering,Wuhan University of Science and Technology,Wuhan 430081,China)
出处
《西北农业学报》
CAS
CSCD
北大核心
2019年第3期452-458,共7页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金(31301675)~~
