摘要
为了提取和利用辣木籽蛋白质,本文通过水酶法优化其提取条件,并探讨其功能性质。以辣木籽为原材料,以蛋白质提取率为指标,首先确定了最佳使用酶为Alcalase碱性蛋白酶,再通过单因素试验考察料液比、时间、温度、酶添加量和pH等因素对蛋白质提取率的影响,在此基础上,利用响应面试验设计优化水酶法提取辣木籽蛋白质的工艺。结果表明,最佳工艺条件为:使用Alcalase碱性蛋白酶,在料液比为1∶10,酶添加量为4.5%,pH为9.0,温度为60℃,时间为4.5 h,此时辣木籽蛋白质的提取率最高为68.23%。在pH为10、温度为55℃时辣木籽蛋白质的氮溶解指数最高;辣木籽蛋白质持水性随着pH的增加而增加,在温度为40℃时持水性最好;在温度为55℃时,辣木籽分离蛋白的吸油效果最明显。
In order to extract and utilize the protein from the seeds of Moringa oleifera ,the extracting condition was optimized by aqueous enzymatic method and the functional properties of protein were also evaluated.Effects of pH,temperature,time and enzyme amount on the extraction rate of protein from the seeds of Moringa oleifera were established by single-factor tests.Protein was extracted by aqueous enzymatic method and Box-Benhnken design and response surface method was used to optimize the extraction process on the basis of single-factor tests.According to this model,the optimal parameters of extracting protein from Moringa oleifera seeds were determined as solvent/material ratio 1 ∶10,extraction temperature 60 ℃,hydrolysis time 4.5 h,enzyme amount 4.5%,pH 9.0 with Alcalase protease.The extraction rate under this optimum condition was 68.23%.The nitrogen solubility index of protein from Moringa oleifera seeds was up to its peak when the process condition was pH 10,a temperature of 55 ℃.The water holding capacity increased as the increasing pH value and was best when the temperature was 40 ℃.The oil absorption ability was most obvious at the temperature of 55 ℃.
作者
王标诗
李雪儿
梁小慧
胡小军
张卫国
江敏
杨娟
张世奇
WANG Biao-shi;LI Xue-er;LIANG Xiao-hui;HU Xiao-jun;ZHANG Wei-guo;JIANG Min;YANG Juan;ZHANG Shi-qi(School of Chemistry and Chemical Engineering,Lingnan Normal University;Engineering and Technology Research Center of Development and Utilization of Moringa oleifera in Guangdong Province,Zhanjiang 524048 ,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2019年第4期587-594,674,共9页
Natural Product Research and Development
基金
广东省自然科学基金(2016A030307018)
中央财政支持地方高校发展专项资金(0003018015)
岭南师范学院自然科学基金(LP1615
LY1810
ZL1817)
关键词
辣木籽
蛋白质
提取
水酶法
响应面法
功能性质
Moringa oleifera seed
protein
extraction
aqueous enzymatic method
response surface method
functional properties
