摘要
目的探索环孢素A(CsA)在骨髓移植患者代谢产物AM1和AM4N与母药CsA的关系,为CsA在临床合理使用提供支持。方法用高效液相色谱-串联质谱法(HPLC-MS/MS)对骨髓移植患者个体内和个体间的全血样本进行CsA,AM1和AM4N血药浓度检测。结果 CsA个体内代谢:AM1与CsA相关性不好(r=0.79),AM4N与CsA和AM1相关性均较好(r>0.9),代谢产物AM1和AM4N含量及比率各异;CsA个体间代谢:个体间CsA和AM1的相关性较差(r=0.59),AM4N和CsA及AM1在个体间不存在相关性(r<0.5),代谢产物AM1和AM4N含量及比率各异。结论 CsA代谢存在个体差异;HPLC-MS/MS法可用于骨髓移植患者CsA血药浓度检测。
Objective To explore the relationship among AM1,AM4Nand CsA in bone marrow transplant patients,and provide support for the rational use of CsA in clinical practice.Methods HPLC-MS/MS method was used to detect the concentrations of CsA,AM1and AM4Nin whole blood samples(both within and between individuals)of bone marrow transplant patients.Results Internal metabolism of CsA:the correlation between AM1and CsA was not good(r=0.79),the correlation between AM4Nand CsA and AM1was good(r>0.9),and the content and ratio of metabolites AM1and AM4Nwere different.Interindividual metabolism of CsA:there was poor correlation between CsA and AM1among individuals(r=0.59),and there was no correlation between AM4N,CsA and AM1among individuals(r<0.5).The content and ratio of metabolites AM1and AM4Nwere different.Conclusion There were individual differences in CsA metabolism.HPLC-MS/MS method can be used to detect the blood concentration of CsA in patients with bone marrow transplantation.
作者
王磊
孙文利
刘瑞
王红春
刘红星
WANG Lei;SUN Wen-li;LIU Rui;WANG Hong-chun;LIU Hong-xing(Hebei Yanda Ludaopei Hospital,Hebei Sanhe 065201,China;Beijing Ludaopei Institute of Hematology,Beijing 100176,China)
出处
《现代检验医学杂志》
CAS
2019年第2期52-55,129,共5页
Journal of Modern Laboratory Medicine
