摘要
为有效区分和鉴别烤后烟叶品种,以卷烟企业常用的11个主要烟草品种初烤烟叶为材料,应用磁珠法提取烟叶基因组,并通过PCR和琼脂糖凝胶电泳构建不同品种的SSR指纹图谱。结果表明,从24对候选SSR引物对中筛选出5对多态性高、稳定性强的引物对,其扩增片段长度在100～300 bp之间,经3%琼脂糖凝胶电泳获得了清晰条带。由5对SSR引物的电泳图谱构建了11个烟草品种指纹图谱,并绘制出鉴定流程图,实现了11个烟草品种的快速区分。本方法简单易行、操作性强,可用于选定范围内的初烤烟叶品种的真实性和一致性判定。
To effectively identify the varieties of cured tobacco leaves, the genomic DNA of cured leaves of 11 varieties commonly used was extracted by magnetic bead method. The fingerprint spectra based on SSR markers were constructed by PCR and agarose gel electrophoresis. The results showed that five primer pairs with high polymorphism and stability were selected from 24 SSR primer pairs. The amplified products of the five primer pairs were 100-300 bp in length and clear bands were obtained via 3% agarose gel electrophoresis. On the basis of electrophoregram of the five primer pairs, the fingerprint spectra of the 11 tobacco varieties were constructed, and the flow chart of variety identification was drawn for rapid differentiation of the 11 tobacco varieties. This method is simple, practical and convenient to identify the varieties of cured tobacco leaves.
作者
孙九喆
杨金初
苏东嬴
王二彬
王君婷
张顺峰
李萌
马林
SUN Jiuzhe;YANG Jinchu;SU Dongying;WANG Erbin;WANG Junting;ZHANG Shunfeng;LI Meng;MA Lin(Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou 450000, China;College of Food Science and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China)
出处
《烟草科技》
EI
CAS
CSCD
北大核心
2019年第3期26-32,共7页
Tobacco Science & Technology
基金
河南中烟工业有限责任公司科技计划项目"黄金叶品牌烟叶品种DNA指纹图谱库建立的应用研究"(ZW2015012)
关键词
初烤烟叶
品种
SSR标记
指纹谱库
Cured tobacco leaf
Variety
SSR marker
Fingerprint spectrum library
