摘要
为比较光合细菌菌剂与沼泽红假单胞菌(Rhodopseudomonas palustris)的生理生态特性,分析了不同初始菌量的菌剂PG和菌株PSB-1对实验水体氨氮(NH_4^+-N)、亚硝氮(NO_2^--N)、硝氮(NO_3^--N)和活性磷(PO_4^(3-)-P)的降解效果,通过高通量测序分析了菌剂PG的优势菌组成及实验结束时水体细菌数量和微生物群落组成。结果显示,菌剂PG组对实验水体的PO_4^(3-)-P、NO_3^--N和NO_2^--N有一定的降解作用,其最大降解率分别为40.98%、28.28%和20.12%。菌株PSB-1组仅对实验水体的NO_2^--N和PO_4^(3-)-P有一定的降解效果,其最大降解率分别为14.19%和9.88%。菌剂PG的主要优势菌为红假单胞菌属(Rhodopseudomonas sp.)。实验7 d后实验组水体细菌数量和微生物群落结构发生变化,水体细菌数量增长,形成以异养细菌为优势菌的菌群结构。结果表明光合细菌菌剂PG对水质因子的降解效果优于沼泽红假单胞菌PSB-1,但与报道的高效光合细菌菌株的降解能力存在一定差距。
To compare the physiological and ecological characteristics of inoculant of photosynthetic bacteria and Rhodopseudomonas palustri, we analyzed the degradation effects of the inoculant PG and the strain PSB-1 with different initial amounts of bacteria on ammonia nitrogen( NH+4-N), nitrite nitrogen(NO2--N), nitrate nitrogen( NO3--N) and phosphate(PO43-P) in experimental water.Then we studied the dominant bacteria composition of inoculant of photosynthetic bacteria PG, as well as the number of bacteria and microbial community composition in experimental water at the end of the experiment by high-throughput sequencing. The results show that the inoculant PG group had certain degradation effects on PO3-4-P, NO3--N and NO2--N in experimental water. The maximum degradation rates of PG for PO3--P,-N and 4 NO-3 NO2--N were 40.98%, 28.28% and 20.12%, respectively. Moreover, the strain PSB-1 group only had certain degradation effect on NO2--N and PO43-P. The maximum degradation rates of PSB-1 were 14.19% and9.88%, respectively. The dominant bacteria of the inoculant PG belonged to Rhodopseudomonas sp.. After 7 d of experiment, the number of bacteria and microbial community structure in the experimental group changed, and the number of bacteria in the water increased, forming a microbial structure with heterotrophic bacteria as the dominant bacteria. It is indicated that the inoculant of photosynthetic bacteria PG has better effect on the degradation of water quality factors than R. palustris PSB-1. However, there is a certain gap between both things and the reported degradation efficiency of photosynthetic bacterial strains.
作者
信艳杰
胡晓娟
曹煜成
徐煜
许云娜
苏浩昌
徐创文
文国樑
李卓佳
XIN Yanjie;HU Xiaojuan;CAO Yucheng;XU Yu;XU Yunna;SU Haochang;XU Chuangwen;WEN Guoliang;LI Zhuojia(College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of South ChinaSea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs,Guangdong Provincial Key Laboratoryof Fishery Ecology and Environment,South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Guangzhou 510300, China;Guangzhou Xinhai Lisheng Biotech Co., Ltd., Guangzhou 510300, China)
出处
《南方水产科学》
CAS
CSCD
北大核心
2019年第1期31-41,共11页
South China Fisheries Science
基金
中国水产科学研究院基本科研业务费专项资金(2017HY-ZD0501)
现代农业产业技术体系建设专项资金(CARS-48)
广东省科技计划项目(2016A020210024)
海南省自然科学基金项目(20163148)
广东省渔港建设和渔业产业发展专项(A201701B06)
广东省促进经济发展专项资金(现代渔业发展用途)省级项目(SDYY-2018-02)
关键词
光合细菌菌剂
沼泽红假单胞菌
氮磷营养盐
微生物群落
高通量测序
inoculant of photosynthetic bacteria
Rhodopseudomonas palustris
nitrogen and phosphorus nutrients
microbial community
high-throughput sequencing
