摘要
背景:在不添加保护剂的情况下,通过适宜冻存方法长期低温保存的多余脂肪组织能否直接二次移植,并达到较好的填充效果,是一项值得研究的课题。目的:应用梯度低温保存的脂肪颗粒作为皮下填充物的移植效果,探讨其临床应用的可行性。方法:将人抽脂获取的脂肪颗粒在不添加保护剂的情况下,经梯度降温置于-80℃冰箱行冷冻保存,实验分组新鲜脂肪、冻存1个月组、冻存3个月组、冻存6个月组。8-10周龄SPF级裸鼠12只,体质量约20 g,由徐州医科大学实验动物中心提供。将各组脂肪颗粒同期植入裸鼠背部皮下,移植后第4,8周取出样本,比较各组移植物大体外观、质量、体积变化;通过细胞提取、MTT检测、苏木精-伊红及免疫组织化学染色,观察比较脂肪组织的成活情况。结果与结论:经梯度低温保存后的脂肪颗粒在移植取出后均有脂肪细胞存活,且胞间可见新生血管生成。其中冻存6个月的脂肪颗粒移植效果甚至优于冻存1、3个月的脂肪。说明通过适宜的冻存步骤,未添加冷冻保护剂的脂肪颗粒经梯度低温保存可安全移植于生物体,并达到较满意的填充效果。
BACKGROUND:In the case of not adding protective agent,whether excessive adipose tissue cryopreserved for long time can be used for the second transplantation and achieve good filling effect is worthy to be investigated.OBJECTIVE:To investigate the transplantation effect of fat particles cryopreserved for different time,and the feasibility of clinical application.METHODS:Without addition of protectant,fat particles were cryopreserved at a final temperature of-80℃.There were four groups:fresh fat,cryopreservation for 1,3,and 6 months groups.Twelve 8-10-week-old SPF nude mice weighting 20 g provided by the Laboratory Animal Center of Xuzhou Medical University were included in this study.After cryopreserved for different time,fat particles were separately implanted into the back of nude mice.Tissue samples were removed at 4 and 8 weeks after transplantation.Appearance,weight,and volume of graft were compared among groups.The survival of fat tissue was determined by MTT assay,hematoxylin-eosin staining and immunohistochemical staining.RESULTS AND CONCLUSION:After transplantation of cryopreserved fat particles,there were surviving adipocytes,and new blood vessels were observed between cells.The transplantation effect of fat particles cryopreserved for 6 months was superior to that of fat particles cryopreserved for 1 and 3 months.Therefore,fat particles cryopreserved for different time without addition of protectant can be transplanted without safety concern,which can obtain satisfactory filling outcomes.
作者
郑万玲
张爱君
王浩
王苹苹
李健华
温敏敏
金培生
Zheng Wanling;Zhang Aijun;Wang Hao;Wang Pingping;Li Jianhua;Wen Minmin;Jin Peisheng(Graduate School,Xuzhou Medical University,Xuzhou 221002,Jiangsu Province,China;Department of Plastic Surgery,the Affiliated Hospital of Xuzhou Medical University,Xuzhou 221000,Jiangsu Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2019年第11期1706-1710,共5页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金(81571901)
项目负责人:金培生~~
