摘要
为研究食源性海洋天然ACE抑制肽的体内降血压效果,以太平洋牡蛎Crassostrea gigas为原料,采用脱盐、二次酶解、超滤等工艺方法,制备相对分子质量3000以下的牡蛎ACE抑制肽,对其抗消化能力、乳化性、乳化稳定性、吸水性、吸油性和溶解性等性质进行研究,并通过雄性原发性高血压大鼠(Spontaneously hypertensive rats,SHR)进行灌胃试验(28 d),研究牡蛎ACE抑制肽的降血压作用。结果表明:用二次酶解法制备的牡蛎肽粉中多肽含量为66.7%,糖分为18.2%,灰分为6.31%,且含有44.28%的必需氨基酸和14.17%的疏水性氨基酸,17种氨基酸组成良好,表明制备的牡蛎ACE抑制肽纯度较高,牡蛎ACE抑制肽具有良好的消化稳定性,其吸油能力比吸水能力强,不同p H(6~10)和温度(50~90℃)下溶解性较好;动物试验结果表明,第21天时牡蛎ACE抑制肽组SHR心脏收缩压(SBP)降到最低值,为(161.56±16.63)mm Hg,之后基本保持恒定且极显著低于空白组(P<0.01),停止灌胃ACE抑制肽6 d内,ACE抑制肽组SHR的心脏收缩压与空白组相比仍表现出极显著性差异(P<0.01)。研究表明,牡蛎ACE抑制肽的降压体制在体内能发挥较好作用并能持续性稳定血压,可作为海洋天然活性肽被开发和利用。
The anti-digestive capacity,emulsifying activity,emulsifying stability,water absorption,oil absorption and solubility were investigated in ACE inhibitory oyster peptides with molecular weight less than 3000 from oyster Crassostrea gigas prepared by desalting,secondary enzymatic hydrolysis,and ultrafiltration and the effect of food-borne natural marine ACE inhibitory peptides on blood pressure in vivo was studied in spontaneously hypertensive rats(SHR)by intragastric administration.The results showed that the oyster peptide powder prepared by secondary enzymatic hydrolysis method had polypeptide content of 66.7%,sugar content of 18.2%,and ash content of 6.31%,indicating that the prepared oyster ACE inhibitory peptide showed high purity,with 44.28%of essential amino acids including 17 perfective amino acid compositions and 14.17%hydrophobic amino acids.Oyster ACE inhibitory peptides had good state in terms of digestion stability,stronger oil absorption capacity than water absorption capacity,and good solubility at different pH(6-10)and temperature(50-90℃).The animal test showed that the minimal systolic blood pressure(SBP)[(161.56±16.63)mm Hg]was observed in SHR exposed to intragastric administration on the twenty-first days after intragastric administration of ACE inhibitory peptides of oysters,significantly lower than that in the control group(P<0.05)afterwards.The findings indicated that oyster ACE inhibitory peptide has a good antihypertensive effect in the body,with stable effect,which can be developed and utilized as a marine natural active peptide.
作者
张可佳
张胜男
祁艳霞
赵前程
李智博
ZHANG Ke-jia;ZHANG Sheng-nan;QI Yan-xia;ZHAO Qian-cheng;LI Zhi-bo(College of Food Science and Engineering,Key Laboratory of Aquatic Product Processing and Utilization of Liaoning Province,Dalian Ocean University,Dalian 116023,China)
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2018年第6期788-794,共7页
Journal of Dalian Ocean University
基金
国家海洋公益性行业科研专项(201505030-4).
关键词
牡蛎
ACE抑制肽
性质
降血压
Crassostrea gigas
ACE inhibitory peptide
characteristic
antihypertensive
