摘要
为研究扇贝裙边ACE抑制肽的结构及其降血压效果,以虾夷扇贝Patinopecten yessoensis裙边酶解液为研究对象,采用凝胶色谱及反相高效液相色谱对ACE抑制肽组分进行分离纯化、ESI MS/MS鉴定和动物试验。结果表明:经Sephadex LH-20分离得到的组分F5和F7具有ACE抑制活性,其半抑制浓度(IC_(50))分别为1.4、1.7 mg/m L;通过两次反相高效液相色谱分离得到F5-3-3,经质谱解析筛选出的肽段Val-Trp(VW)具有ACE抑制活性,其IC_(50)为86.9μmol/L;用扇贝酶解液经超滤获得的相对分子量小于3000的组分灌胃雄性原发性高血压大鼠(SHR)28 d,灌胃第9天时,SHR的心脏收缩压(SBP)达到172 mm Hg,之后心脏收缩压基本保持恒定且极显著低于空白对照组(P<0.01),停止灌胃7 d内,多肽处理组SHR的心脏收缩压与空白对照组相比仍表现出极显著性差异(P<0.01)。研究表明,扇贝裙边酶解产物具有较好的降血压效果,可作为研发降压药物或保健食品的良好原料。
The angiotensin convert enzyme peptides(ACE)inhibitor peptides were purified from enzymatic hydrolysate of soft part excluding adductors of yesso scallop Patinopecten yessoensis by gel filtration chromatography and reversed-phase high-performance liquid chromatography(RP-HPLC),and then the structure and hypertensive activity of the ACE inhibitor peptides were studied.The fractions F5and F7 separated by Sephadex LH-20were shown to inhibit ACE,with 50% inhibitory concentration(IC50) of 1.4mg/mL for F5 and 1.7mg/mL for F7.The F5-3-3 was derived from F5-3 with RP-HPLC for twice,and the peptide Val-Trp(VW)identified by mass spectrometry(MS)had ACE inhibition,with IC50 of 86.9μmol/L.Then the enzymic hydrolysate with molecular weight of below 3000 obtained by filtration was gavaged to spontaneously hypertensive rat(SHR) for 28 days when very significantly decrease in systolic blood pressure(SBP)was observed in SHR(P<0.01),even very significantly lower(systolic pressure of 172mmHg)than that in the control group 9 days after intragastric administration(P<0.01).The findings indicate that the enzymic hydrolysate from soft part excluding adductors of yesso scallop has a role in reduction in blood pressure and becomes a candidate for development of hypoglycemic drugs and health food.
作者
李晶晶
尤海琳
张亚飞
李敏
赵慧
胡建恩
卢航
LI Jing-jing;YOU Hai-lin;ZHANG Ya-fei;LI Min;ZHAO Hui;HU Jian-en;LU Hang(College of Food Science and Engineering,Dalian Ocean University,Dalian 116023,China)
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2018年第6期782-787,共6页
Journal of Dalian Ocean University
基金
国家海洋公益性行业科研专项(201505029-3)
关键词
扇贝裙边
ACE抑制肽
分离纯化
降血压
soft part excluding adductor
ACE inhibitor peptide
isolation and purification
hypotensive activity
