摘要
目的探讨地衣芽孢杆菌对非酒精性脂肪性肝病(NAFLD)的干预作用和对肠黏膜通透性的影响。方法 Wistar大鼠随机分为正常(Con)组、模型(Mod)组、地衣芽孢杆菌低(BLL)、高(BLH)剂量组。大鼠用高脂饲料喂养复制NAFLD模型,第8周开始灌胃给药,末次给药后测定血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、总胆固醇(TC)、三酰甘油(TG)、二胺氧化酶(DAO)、内毒素(ETX)及肝匀浆中肿瘤坏死因子-α(TNF-α)的水平,免疫组化观察小肠中Occludin和ZO-1的表达。结果与Con组比较,Mod组血清中各生化指标水平明显升高,肝组织中TNF-α含量增加,小肠中Occludin和ZO-1蛋白表达明显降低(P <0.05);与Mod组相比,BLL、BLH组大鼠血清中ALT、AST、TC、TG、DAO及ETX含量明显降低,肝中TNF-α含量减少,肝组织病理学明显改善,小肠中Occludin和ZO-1的表达增加(P <0.05)。结论地衣芽孢杆菌可缓解NAFLD大鼠肝脏脂肪变性和炎症,其机制可能与增加小肠紧密连接蛋白的表达有关。
Objective To investigate the effect of Bacillus licheniformis on non-alcoholic fatty liver disease(NAFLD)and the intestinal permeability in ratsMethods Wistar rats were randomly divided into normal control group(Con),model group(Mod),low(BLL)and high dose group(BLH)of Bacillus licheniformis.Animal models of NAFLD were induced with high fat diet. From the 8 th week,different drugs were administrated via gavage. After the last administration,the level of alanin aminotransferase(ALT),aspartate aminotransferase(AST),total cholest(TC),triglycerides(TG),diamineoxidase(DAO)endotoxin(ETX) in serum and the contents of tumor necrosis factor-α(TNF-α)in liver homogenate were detected. Pathological changes of liver tissue were observed. The expression of Occludin and ZO-1 in small intestine was analyzed by immunohistochemical method.Results Compared with those in the Con group,the levels of ALT,AST,TC,TG,DAO,ETX in serum and the contents of TNF-α in liver in Mod group were significantly increased. The expression of Occludin and ZO-1 in small intestine was reduced(P < 0.05). Compared with those in the Mod group,the levels of ALT,AST,TC,TG,DAO,ETX in serum and the contents of TNF-α in liver in BLL and BLH groups were significantly reduced(P < 0.05). The liver pathologic changes were improved and the expression of Occludin and ZO-1 in small intestine in BLL and BLH groups was increased.Conclusion Bacillus licheniformiscan reduce the degree of hepatic steatosis and inflammation and the mechanism may be related with the up-regulation of Occludin,ZO-1 and the improvement of intestinal barrier function.
作者
宋献美
吴晓东
石科
许波
SONG Xianmei;WU Xiaodong;SHI Ke;XU Bo(Department of Microbiology and Immunology,Henan Medical College,Zhengzhou 451191,China)
出处
《实用医学杂志》
CAS
北大核心
2018年第24期4056-4059,共4页
The Journal of Practical Medicine
基金
河南省医学科技攻关计划(编号:201503234)
关键词
地衣芽孢杆菌
非酒精性脂肪肝病
肠黏膜通透性
紧密连接
bacillus licheniformis
non-alcoholic fatty liver disease
intestinal permeability
tight junction
