摘要
目的探讨姜黄素对非酒精性脂肪性肝病(NAFLD)大鼠肠黏膜肌球蛋白轻链激酶(MLCK)的影响。方法 35只SD大鼠随机分为3组,正常对照组(NC)10只、高脂饲料喂养组(HF)15只、姜黄素组(Cur)10只,后两组予高脂饲料喂养16周,其中Cur组在高脂饲料喂养8周后,予姜黄素灌胃200 mg/(kg·d),每天1次,共8周。检测大鼠血ALT、AST、LPS、DAO水平及肠黏膜MLCK的表达;观察肝脏病理学及肠黏膜紧密连接。结果与NC组比,HF组大鼠血ALT、AST、LPS及DAO均明显升高(P<0.05);与HF组比,Cur组大鼠血ALT、AST、LPS及DAO均明显下降(P<0.05)。HF组大鼠肝细胞脂肪变性明显,Cur组肝细胞脂肪变减轻。HF组紧密连接间隙较NC组明显增宽(P<0.05),Cur组紧密连接间隙较HF组变窄(P<0.05)。HF组MLCK表达较NC组明显升高,Cur组阳性染色较HF组减弱(P<0.05)。结论姜黄素能够通过下调NAFLD大鼠肠黏膜MLCK的表达,改善肠黏膜紧密连接结构。
Objective To investigate the effects of curcumin on myosin light chain kinase（MLCK）in theintestinal mucosa of rats with nonalcoholic fatty liver disease（NAFLD）. Methods 35 SD rats were randomly di-vided into the normal control group,the high fat group and the curcumin group. The high fat group,curcumingroup were given high fat diet for 16 weeks. After 8 weeks of high fat feeding in the curcumin group,the rats weregavaged with 200 mg/（kg·d）curcumin for 8 weeks. The levels of ALT,AST,LPS and DAO in blood and expres-sion of MLCK in the intestinal mucosa were detected. The changes of liver pathology and tight junction（TJ）of theintestinal mucosa were observed. Results Compared with the control group,the blood levels of ALT,AST,LPSand DAO in the high fat group were obviously increased（P 0.05）;Compared with the high fat group,the bloodlevels of ALT、AST、LPS and DAO in the curcumin group were obviously decreased（P 0.05）. In the high fatgroup,hepatocellular steatosis was obvious,while in the curcumin group hepatocellular steatosis was decreased.TJ was disrupted in the high fat group,and the intercellular space was larger in the TJ group than the control group（P 0.05）. The intercellular space was narrower in the curcumin group than the high fat group（P 0.05）. The expression of MLCK in the high fat group was significant higher than that in the control group（P 0.05）. The pos-itive staining in the curcumin group was significant lower than that in the high fat group（P 0.05）. Conclusion Curcumin can ameliorate hepatic steatosis by downregulating expression of MLCK in the intestinal mucosa of ratswith NAFLD,improving TJ structure of the intestinal mucosa.
出处
《实用医学杂志》
CAS
北大核心
2017年第20期3363-3367,共5页
The Journal of Practical Medicine
基金
河北省医学科学研究重点课题计划(编号:20170275)
关键词
非酒精性脂肪肝
姜黄素
肌球蛋白轻链激酶
肠黏膜
nonalcoholic fatty liver
curcumin
myosin light chain kinase
intestinal mucosa
