摘要
目的评价人乳头瘤病毒(HPV) E6/E7 mRNA检测在年轻女性(≤30周岁)宫颈癌筛查及宫颈疾病诊治中的应用效果。方法采用前瞻性随机双盲对照研究法,选择2015年1月至2016年12月西安医学院第二附属医院收治的122例行宫颈薄层液基细胞学(TCT)筛查为意义不明的非典型鳞状上皮细胞(ASCUS)/低度鳞状上皮内病变(LSIL)的年轻女性(≤30周岁)患者随机分为对照组和观察组,每组各61例。对照组行HPV DNA检测,观察组行HPV E6/E7 mRNA检测,比较两组患者的病变预测准确率及患者满意率。结果对照组中HPV DNA检测阳性32例,检出率为52. 5%;观察组中HPV E6/E7 mRNA检测阳性18例,检出率为29. 5%;观察组的检测阳性率低于对照组(χ~2=26. 339,P <0. 05),组间比较差异具有统计学意义(P <0. 05)。宫颈上皮内瘤变(CIN)Ⅱ级以下患者,观察组的检测阳性率低于对照组(P <0. 05),CINⅡ级以上患者,观察组的检测阳性率与对照组比较差异无统计学意义(P> 0. 05);观察组的阴道镜转诊+活检率低于对照组,满意率高于对照组(P <0. 05)。结论相较于常规的HPV DNA检测,HPV E6/E7mRNA检测的预测准确性更高、阴道镜转诊+活检率更低、患者满意度更佳,具有较高的临床应用价值。
Objective To evaluate the efficacy of human papillomavirus(HPV)E6/E7 mRNA detection for screening,diagnosis and treatment of cervical cancer in young women(less than 30 years old).Methods By using prospective randomized double-blind controlled study,122 routine cervical thin layer liquid based cytological(TCT)screening for unknown atypical squamous cells(ASCUS)/low-grade squamous intraepithelial lesions(LSIL)in young women(30 years of age or less)patients were randomly divided into two groups,control group(n=61)for line of HPV DNA testing,observation group(n=61)for HPV E6/E7 mRNA detection.Compared two groups of patients with lesion prediction accuracy,colposcopy plus biopsy referral rate and patients'satisfaction.Results In control group,32 cases were positive in HPV DNA detection,the detection rate was 52.5%,and 18 cases with HPV E6/E7 mRNA detection in observation group were 29.5%,and the positive rate of observation group was lower than that of control group(χ^2=26.339,P<0.05),and the difference between these 2 groups was statistically significant(P<0.05).The positive rate of observation group was lower than that of control group(P<0.05),and the positive rate of observation group was lower than that of control group.The difference in positive rate of observation group was not statistically significant in comparison with that of control group(P>0.05).The positive rate of biopsy in observation group was lower than that of control group,and the rate of satisfaction was higher than that of control group(P<0.05).Conclusion In comparison with conventional HPV DNA detection,HPV E6/E7 mRNA detection has higher accuracy for prediction,lower colposcopy referral biopsy rate,with better satisfaction of patients,and higher clinical significance for application.
作者
岳晚侠
吴利侠
蒙树勇
YUE Wan-xia;WU Li-xia;MENG Shu-yong(Department of Pathology, Second Affiliated Hospital of Xi'an Medical College, Xi'an, Shaanxi 710038, China;Department of Oncology, The Second Affiliated Hospital of Xi'an Medical University, Xi'an Shaanxi 710038, China)
出处
《临床和实验医学杂志》
2019年第1期66-68,共3页
Journal of Clinical and Experimental Medicine
基金
陕西省卫生厅科研基金项目(编号:2015JM4029)
