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文昌鱼哈氏窝匀浆使蟾蜍足细胞释精的超微结构研究

Ultrastructural study of sertoli cell of toad during induced spermiation by injection of homogenate of Hatschek′s pit of amphioxus
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摘要 本文报道了文昌鱼哈氏窝匀浆刺激蟾蜍足细胞释放精子过程所发生的超微结构变化,进一步证实这种匀浆含有类似脊椎动物促黄体生成素活性物质。 The paper reports that the promotion of spermiation of toad by the homogenate of Hatschek's pit was examined under light and electron microscopic observations. The mechanism of spermiation from the Sertoli cell was considered as follows: firstly, the homogenate of Hatschek's pit stimulates to increase the water content in toad testis; secondly, the swelling and expanding of the smooth endoplasmic reticulum of the Sertoli cell results in the formation of large lacunae, and finally, the sperms were released into the lumen of seminiferous tubules. At the same time, the mitochondria in the cytoplasm of the Sertoli cell were fully developed, and the secondary lysosomes were also present in the cytoplasm. These mechanism is similar to LH or HCG which induces the ultrastructural change of the Sertoli cell during toad spermiation.The results further demonstrate that the homogenate of the Hatschek's pit of amphioxus may contain an active substance which may be similar to LH of vertebrates.
作者 方永强 王龙
出处 《台湾海峡》 CAS CSCD 1991年第1期59-62,103,104,共6页 Journal of Oceanography In Taiwan Strait
关键词 文昌鱼 喻氏窝匀浆 蟾蜍 细胞 精子 Lancelet sertoli cell ultrastructure
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  • 1方永强,汪敏.丘脑下部促黄体素释放激素类似物(LRH-A)的作用机制 Ⅲ.对罗非鱼脑垂体促性腺激素分泌细胞超微结构的影响[J]动物学报,1983(02).
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  • 3方永强,汪敏,许瑞安.丘脑下部促黄体素释放激素类似物(LRH-A)的作用机制Ⅰ.脑垂体组织生理学的研究[J]动物学报,1981(03).
  • 4Loan T. Tjoa,Dr. Ulrich Welsch. Electron microscopical observations on K?lliker’s and Hatschek’s pit and on the wheel organ in the head region of Amphioxus (Branchiostoma lanceolatum)[J] 1974,Cell and Tissue Research(2):175~187
  • 5方永强,王龙.文昌鱼LH-RH的初步分离及其生物活性的测定[J].动物学报,1989,35(3):339-340. 被引量:3
  • 6张致一,朱益陶,陈大元.促黄体素(LH)在文昌鱼哈氏窝中的免疫细胞化学定位[J].科学通报,1982(15):946-947. 被引量:8

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