摘要
采用猴13只,其中妊娠8周猴2只。应用1甲基1,2,5,64氢吡啶(MPTP)诱发猴帕会森氏病模型,将同种胚胎中脑腹侧部植入猴PD模型的尾状核内。术后观察6个月,病理症状、肌电图、旋转行为等与移植前无明显改善。MRI检查可见移植区有点块状低信号影(T_(1)像)。组织病理学检查,移植1(1/2)月后,移植物内只有少数神经元存活,3个月后移植物内神经元消失,6个月后移植部位仅存在胶质疤痕,大量吞噬细胞及淋巴球浸润。显示慢性排异现象尚未完全终止。实验表明,脑组织移植后,对病理症状的改善不明显,移植的神经元未能在宿主脑内长期存活。
Thirteen adult monkeys and their two 8 weeksembyo were used in the experiment.By injectingMPTP into unilateral internal carotid artery,ipsila-teral parkinsonism model was performed in monkey.One month later a piece of ventral mesencephalonwas removed from embryos and then transplantedinto the head of caudate nucleus of animal model.After operation careful observed these animals onpathological syndromes,EMG and rotationalbehavior.There were no any improvment thanbefore.MRI images showed small area of lowsignal(T(?) sign)over the tissue transplanted part.Histopathological investigations,only a few numberof transplanted cells can be found to survive oneand half month and after three months,mosttransplanted neurons disappeared.Six months laterthere were gliosis over that area.Macrophage andlymph cells presented,indicating chronic rejectionnot completely ceased yet.The results showedthat fetal brain tissue had no significent improvementon the pathological signs.Transplanted cells couldnot survived for a longer time in the host brain.
出处
《中华神经外科杂志》
CSCD
北大核心
1991年第3期218-221,I008,共5页
Chinese Journal of Neurosurgery
