摘要
裸小鼠不仅对研究人体肿瘤细胞的肿瘤发生发展,而且对研究肿瘤细胞与宿主环境之间的相互关系均可提供有价值的模型.人体恶性肿瘤在裸小鼠体内能否生长,生长后能否发生侵袭和转移,除了肿瘤本身的生物学特性外,肿瘤移植部位的环境因素对肿瘤的生长、侵袭和转移也起重要的作用.本实验进一步观察和验证移植途径对肿瘤生长和侵袭能力的影响.
In the present study, the growth and invasion of human lung adenocarcin-oma cells (LTEP-a-2) transplanted into different sites (s.c. and footpad) and using different methods (including inoculation of tumor cell suspension and tumor fragment) in 12 nude mices were examined. The animals were divided into two groups; namely, subcutaneous ineculation(6 nude mice) and footpad inoculation (6 nude mice). Two transplantation sites were selected in each mouse; i.e. the tumor cell suspention was implanted into one site, and the tumor fragment was implanted into the opposing site of the same mouse.In the subcutaneous inoculation group, tumor growth (100%) was accompanied by no invasive behavior (in-ciluding suspension and fragment). In the footpad inoculation group, 3/6 cases of inoculation by suspension saw tumor growth with invasive behavior; but in the case of tumor fragment transplantation no tumor growth was found.One murine sarcoma was found in the tissues surrounding the human lung adenocarcinoma cells. The mechanism of formation of this murine sarcoma s discussed.Our results show that human tumor growth in nude mice is obviously influenced by the site of inoculation.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1991年第6期459-462,T026,共5页
Acta Academiae Medicinae Sinicae
