摘要
对处于不同生长阶段银鲫的蛋白酶、脂肪酶、淀粉酶、纤维素酶的活性分布进行了初步研究。结果表明 :在鱼苗、鱼种、幼鱼、成鱼和亲鱼 5个生长阶段 ,胰蛋白酶和脂肪酶活性基本保持不变 ,纤维素酶活性呈上升趋势 ,淀粉酶活性从鱼苗到成鱼不断提高 ,但在亲鱼阶段降低。胰蛋白酶和淀粉酶活性分布均以中肠最大 ,纤维素酶活性则在肝胰脏中最大。在肝胰脏、前肠、中肠、后肠 ,胰蛋白酶的最适pH值分别是 7.5、7.5、8.5、8.0 ;脂肪酶的最适pH值均为 7.5。淀粉酶的最适pH值均为 6 .5 ;纤维素酶在肝胰脏的最适pH值是 4.8,在前肠、中肠、后肠最适pH值均有两个峰值 ,分别为 3.6和 4.8。
The activity distribution of trypsin, lipase,amylase and cellulase in Carassius auratus glibelio was examined. The activity of cellulase was increased, and those of lipase and trypsin remained at similar levels during fry, fingerling, juvenile, mature and reproductive stages. The amylase activity was increased from fry to mature stages then decreased at reproductive stage. The activities of trypsin and amylase are higher in intestine sectionⅡ and the cellullase activity is higher in hepatopancreas. The optimum pH values of hepatopancreas ,foregut,midgut,hindgut for the trypsin are 7.5,7.5,8.5,8.0 respectively, and for the lipase are 7.5,and for the amylase are 6.5. The optimum pH value of hepatopancreas for the cellulase is 4.8, and there are two optimum pH values of intestine for the cellulase 3.6 and 4.8. These results showed that different pH values were conductive to different digestive enzymes.
出处
《上海水产大学学报》
CAS
CSCD
2002年第3期193-198,共6页
Journal of Shanghai Fisheries University
关键词
银鲫
消化酶
活性分布
PH值
酶活性
生长阶段
器官
Carassius auratus glibelio
digestive enzyme
activity distribution
pH value
