摘要
用酶水解鸡肉 ,通过单因素实验确定胰蛋白酶 1、中性蛋白酶、木瓜蛋白酶、酸性蛋白酶、碱性蛋白酶、胰酶 2、胃蛋白酶水解鸡肉的最适条件 ,并确定在它们的最适条件下 ,胰蛋白酶和木瓜蛋白酶是单酶水解较好的酶。在双酶水解中 ,发现胰蛋白酶 1与酸性蛋白酶在各自最适条件下组合水解鸡肉水解率最高 ,进一步确定双酶水解的条件为胰蛋白酶先在 5 0℃ ,p H8.5 ,加酶量 40 0 0 U/ g蛋白质 ,固液比 1∶ 4,水解 3h,后酸性蛋白酶在 45℃ ,p H2 .5 ,加酶量 40 0 0 U/ g蛋白质水解 2 h。在这种组合下酶解 ,水解产物的水解度为 40 .5 % ,蛋白回收率为 81.0 %
In this paper,the research of the chicken protein hydrolysate by the enzymatic hydrolysis is studied.The optimum condition for trypsin,neutral protease,papain,acid protease,alkaline protease,pancreatin to hydrolysis are determined by monofactorial experiments,and it come to a conclusion that trypsin and papain have much more hydrolysis effciency.Combined hydrolysis of double enzymes is also studied.In double enzyme hydrolysis,we find that when trypsin and acid protease hydrolyze chicken together at their optimum condition respectively,the hydrolysis degrree is highest.Then we make further work to determine the condition of double enzyme hydrolysis,which is trypsin hydrolysis for 3 hours at 50℃,pH8.5,enzyme amount 4 000 U/g protein,solid/fluid 1∶4 and then acid protease hydrolysis for 2 hours at 45℃,pH2.5,enzyme amount 4 000 U/g protein.The degree of hydrolysis and protein recovery of the hydrolysis are 40.5% and 81.0%,respectively.
出处
《天津轻工业学院学报》
2002年第3期11-13,共3页
Journal of Tianjin University of Light Industry
关键词
鸡肉
蛋白水解液
蛋白酶
双酶水解
protease
double enzyme hydrolysis
chicken protein
hydrolysate
