摘要
通过硫酸铵分级沉淀 ,CM -SephadexC - 2 5 ,DEAE -SephadexA - 5 0 ,POROS 2 0HQ离子交换色谱分离方法 ,从黑曲霉发酵粉中分离纯化了一种新的内切 β -葡聚糖苷酶 .该酶在还原条件下分子量为 39.2kD ,最适pH为 4.0 ,最适温度为 5 5℃ ,Km 为 7.41mg mL .
A novel cellulase was isolated and purified to homogeneity from a commercial Aspergillus niger cellulase preparation by a combination of ammonium sulfate and three steps of ion exchange chromatograph of CM-Sephadex C-25, DEAE-Sephadex A-50 and POROS 20 HQ. The molecular weight of the purified enzyme is 39.2?kD under reduced conditions by SDS-PAGE; the optimal pH of the enzyme is 4.0, and the optimal temperature is 55?℃, its K m is 7.41?mg/mL.
出处
《福州大学学报（自然科学版）》
CAS
CSCD
2002年第5期636-640,共5页
Journal of Fuzhou University(Natural Science Edition)
