摘要
用Fmoc固相多肽合成的方法手工合成了一种ω 芋螺毒素MVIIA和虎纹捕鸟蛛毒素 I的嵌合体HM 1 2 2 7,经过RP HPLC纯化和氧化复性 ,小鼠隔神经 隔肌标本的阻断实验显示此嵌合体能实现二硫键完全配对与稳定折叠并具有微弱的神经毒活性 .实验结果表明 ,ω CtxMVIIA和HWTX I的分子框架稳定 ,部分序列交换后仍能实现二硫键配对与折叠 。
A chimera polypeptide of ω-Ctx MVIIA and HWTX-I has been synthesized through Fmoc solid-phase peptide synthesis. After oxidative renaturation, the chimera peptide was purified by reversed phase HPLC. The experiment of isolated mouse phrenic nerve-diaphragm showed that this polypeptide has low neuronal activity to inhibit the neuro-muscular transmission. The result demonstrated that the chimera molecule can reach disulfide bonds pairing and folding and the structural motif of both HWTX-I and ω-Ctx MVIIA is promising for protein engineering.
出处
《晓庄学院自然科学学报》
EI
CAS
北大核心
2002年第3期68-71,共4页
Journal of Natural Science of Hunan Normal University
基金
国家 8 63高技术发展计划资助项目 (1 0 3 1 3 0 1 0 6)
