摘要
目的 :探讨脾虚证与胆囊收缩素 (CCK)的关系。方法 :用放射免疫测定 (RIA)法对脾虚造模 7d和 14 d大鼠血浆、下丘脑、十二指肠、空肠中 CCK含量进行测定 ,同时用链酶亲和素 -过氧化酶复合物技术 (SABC)法对十二指肠粘膜和下丘脑组织中 CCK内分泌细胞进行染色。结果 :造模 7d大鼠模型组织、血浆中 CCK含量升高 ,十二指肠内分泌 (I)细胞强阳性细胞率升高 ,下丘脑 CCK神经元数升高 (P <0 .0 1,<0 .0 5 ) ;造模 14 d下丘脑、胃窦CCK含量升高 ,十二指肠、空肠和血浆中 CCK含量下降 ,I细胞数、强阳性细胞率及下丘脑 CCK神经元数均升高(P <0 .0 1,<0 .0 5 )。结论 :CCK含量及其内分泌细胞的形态和数量在脾虚证病程中呈动态改变。
Objective: To investigate the relationship between spleen deficiency syndrome (SDS) and cholecystokinin (CCK). Methods: Contents of CCK in rats' plasma, hypothalamencephalon, duodenum and jejunum were measured by using radioimmunoassay 7 and 14 days after rat SDS model was made. At the same time, CCK endocrine cells of duodenum mucosa (I cells) and tissues in hypothalamencephalon were stained with the method of SABC.Result: Seven days after rat SDS model was made, as compared with those in the controls, the percentage of strong positive I cells was increased (P< 0.01 ); the number of CCK neurons in hypothalamencephalon was increased (P< 0.01 ); CCK in both tissues and plasma showed higher levels than that in the controls (P< 0.01 ). Fourteen days after rat SDS model was made, as compared with those in the controls, the number of CCK neurons was increased (P< 0.05 ), the number of I cells and the percentage of strong positive I cells was increased (P< 0.01 ), CCK levels were higher in stomach and hypothalamencephalon and lower in plasma, duodenum and jejunum (P< 0.01 ). Conclusion: The contents of CCK and the morphology and number of the I cells and CCK neurons in hypothalamencephalon varied with the development of spleen deficiency.
出处
《中国中西医结合消化杂志》
CAS
2002年第5期262-264,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
国家自然科学基金资助项目 (No.39770 886
396 70 86 5 )
