摘要
利用免疫组织化学定位方法和Westernblot技术 ,对长途运输试验猪肝脏中 5种不同分子量的热应激蛋白HSP70 、HSP72 、HSP86、HSP90 和HSP2 7进行定位和表达。所有长途运输后应激猪和对照猪肝脏组织中均同时检测到了上述 5种HSP。肝细胞胞浆和胞核均显示阳性信号 ,但HSPs在肝细胞中的定位有差异 ,大多数HSP70 、HSP72 和HSP2 7主要分布于肝细胞胞浆内 ,而HSP90 和HSP86阳性颗粒在核内的分布比其在胞浆中的分布更为明显。肝小叶周边区有明显颗粒变性和脂肪变性的肝细胞中HSPs的阳性染色信号较弱。 6h的长途运输应激后 ,与对照组相比 ,HSPs在肝脏组织中的表达量存在差异 ,表现为HSP70 家族的HSP70 和HSP72 的表达没有明显的升高 (P >0 .0 5 ) ,HSP90 家族的HSP90 的表达出现极显著的增加 (P <0 .0 1) ,然而 ,HSP90 家族的HSP86和小分子量家族的HSP2 7的表达量呈现明显的下降 (P <0 .0 5 )。
To demonstrate the distribution and expression of heat shock proteins (HSPs) in the liver of transport stressed pigs, five kinds of HSPs, namely HSP 70, HSP 72, HSP 86, HSP 90 and HSP 27 were determined after a long distance transport using immunohistochemistry and Western blot. All five HSPs were regularly detected in the livers of both the transported and the control group. Both nuclear and cytoplasmic labeling was present in hepatic cells, however localization of HSPs was quite different in hepatocytes. The majority of HSP 70, HSP 72 and HSP 27 appeared to predominate in cytoplasm of the hepatocytes. Nuclear signals of HSP 90 and HSP 86 were most prominent in hepatocytes in which immunostaining in the cytoplasm was relatively weak compared with that in the nucleus. There was subjectively lower staining in the edge of hepatic lobule, and lower numbers of HSPs labeled hepatic cells corresponded to light microscopic lesions. The obvious increase of some HSPs were detected in liver, especially the HSP 90 (P<0.01), which belongs to the HSP 90 family. However, the obvious reductions of HSP 86 (P<0.05), which belong to the HSP 90 family and HSP 27 (P<0.05), which belongs to small families, were detected in stressed pig liver after long distance transportation in six hours.
出处
《中国农业科学》
CAS
CSCD
北大核心
2002年第9期1130-1133,共4页
Scientia Agricultura Sinica
基金
国家自然科学基金 ( 39770 5 6 9
30 170 6 82 )
中德农业科技合作项目
