摘要
目的 :探讨人乳头状瘤病毒 (HPV)感染在本地区食管癌病因学中的作用及HPV检测在食管癌防治中的意义。方法 :取食管癌手术切除标本 5 0例 ,纤维胃镜下钳取非癌病员正常食管粘膜标本 3 0例 ,分别冰冻切片 ,采用免疫组化ABC法检测HPV ;食管癌标本行常规病理检查 ,分为高、中、低分化癌。结果 :正常食管粘膜及食管癌组织中HPV阳性率差异显著 (P<0 .0 5 )。高、中、低分化癌中HPV阳性率组间差异无显著性。HPV阳性信号分布于细胞浆内 ,阳性信号分布特征与肿瘤分化程度有关。结论 :HPV感染为本地区食管癌发病的一个高危因素 ,HPV检测为食管癌的免疫治疗及预防提供了新的线索。
Objective:To explore the effect of human papilloma virus(HPV) infection on cause of esophageal cancer in Huaian area and explore significance of HPV detection on the prev ention and therapy.Methods:we colle cted 50 esophageal cancer samples which were resected by opera-tion and 30 samples of normal esophageal mucosa which were fetched in patients without tumor by fiber endoscope.The samples of the two groups of were freezed and sliced inspectively,and immun-ohistochemical ABC method was used to detect HPV in thesesamples.Esophageal cancer samples were examined by routine pathological method,which were distinguished into well,mode-rate and poor differentiated cancer.Results:The positive ratios for HPV among normal mucosa samples and among esophageal cancer samples were 0.00% and 74.00% inspectively,There was evident difference between the two ratios(χ 2 test).When the positive ratios for HPV were compared betmeea groups of well,moderate and poor differentiated cancer,there was no evident difference.Positive signals of HPV antigen were distributed over cell plasm,and the distribution characteristic of positive signals related to differentiated degree of tumor cell.Conclusion:The infe ction of HPV is a high risk factor on cause of esophageal cancer in Huaian area.The detection of HPV provides a new clue for immunological therapy and prevention of esophageal cancer.
出处
《江苏临床医学杂志》
2002年第4期334-336,共3页
Journal of Jiangsu Clinical Medicine
