摘要
目的探讨硫酸铝的发育毒性及其发生机制。方法孕8.5d昆明小鼠胚胎于体外培养系统中给予不同剂量的硫酸铝 (Al3 +浓度0.6 ,0.9,1.2 ,3.0 ,9.0μg/ml) ,培养48h后 ,对胚胎进行Maele_Fabry形态评分 ,测量卵黄囊直径 ,胚胎体长及头长 ,胚胎干重 ,并应用DTNB分光光度法、DPH荧光偏振技术 ,观察硫酸铝作用下胚胎组织谷胱甘肽(GSH)和卵黄囊细胞膜脂质流动性的改变。结果当培养液中Al3 +剂量为3.0μg/ml时 ,胚胎生长发育和器官形态分化明显被抑制 ,并使畸形胚胎发生率明显升高 ,主要有神经管未闭 ,脑畸形和体屈异常 ;6.0μg/ml时则可致胚胎组织GSH活性和卵黄囊细胞膜脂质流动性显著降低。上述效应均表现出了一定的剂量_效应 (反应 )关系。结论硫酸铝有潜在的致畸性和胚胎毒性 。
Objective To explore the developmental toxicity of aluminum sulfate and its mechanism. Methods 8.5-day-old embryos of Kunming mice were explanted and cultured in a whole embryo culture system with Al 3+ concentrations of 0.6, 0.9, 1.2, 3.0, 6.0, 9.0 μg/ml for 48 h. Each viable embryo was evaluated using Maele-Fabry scoring system, and visceral yolk sac diameter, crown-rump and head length, and embryo dry weight were measured, as well as GSH activity in embryonic tissue by using 5,5-dithion-bis-2-nitrobenzoic acid (DTNB), and membrane lipid fluidity of visceral yolk sac cell by DPH fluorescence polarization technique. Results Al 2(SO 4)3 at Al 3+ concentration of 3 μg/ml resulted in significant inhibition of development of embryos and differentiation of organs, and increasing prevalance rate of abnormal embryos including open neural tube, small head abnormality and deficit in flexion. At Al 3+ concentration of 6.0 μg/ml, the activity of GSH and the membrane lipid fluidity of visceral yolk sac decreased significantly. In a certain degree, the dose-effect(response) relationship were observed in the above hazardous effects induced by Al 2(SO 4)3. Conclusion Al 2(SO 4)3 presented potential teratagenicity and embryotoxicity, which might be associated with the decreases of the membrane lipid fluidity of visceral yolk sac and the activity of GSH both induced by Al 2(SO 4)3.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2002年第5期374-376,共3页
Journal of Environment and Health
