摘要
以拟南芥野生型 (wt)和ABA合成缺失突变体 (aba3)为材料 ,采用表皮生物分析法研究了NaCl胁迫条件下 ,ABA和H2 O2 在气孔关闭中的作用 .结果表明 :10 0mmol L的NaCl可有效诱导wt气孔关闭 ,而对aba3的气孔运动无明显影响 ;10 - 2 mmol L的H2 O2 处理或 10 0mmol L的NaCl与 10 - 2 mmol LABA共处理可有效诱导wt与aba3的气孔关闭 ,且幅度类似 ;H2 O2 的专一性清除剂CAT可部分逆转NaCl处理及NaCl与ABA共处理引起的气孔关闭 .因此推测 ,NaCl胁迫条件下 ,植物保卫细胞内ABA浓度升高 ,诱导H2 O2 的产生 。
The roles of ABA and H 2O 2 in NaCl induced Stomatal Closure were studied by using wild type (wt) and ABA deficient mutant (aba3) of Arabidopsis thaliana. It is found that 100mmol/L NaCl can effectively induce stomatal closure of wt, but has slight effect on aba3; 10 -2 mmol/L H 2O 2 or 100mmol/L NaCl and 10 -2 mmol ABA together can induce stomatal closure of both wt and aba3 at similar significant level. Further, the stomatal closure induced by NaCl or by NaCl and ABA together are abolished partly by addition of 20U/mL CAT, an H 2O 2 scavenger. The results suggest that H 2O 2 is involved in ABA induced stomatal closure in Arabidopsis under salinity stress.
出处
《河南大学学报（自然科学版）》
CAS
2002年第3期29-32,共4页
Journal of Henan University:Natural Science
基金
河南省科技厅自然科学基金项目 (0 0 4 0 1130 0 )
