摘要
为了解滚压泵长时间转流对红细胞脆性度和形态学的影响。我们采用 2种滚压泵 Polystan小儿泵 (A组 )和 COBE泵 (B组 )分两组作 5次、每次 16 h离体转流实验。测定项目 :红细胞脆性度、红细胞电镜扫描观察和游离血红蛋白测定。血标本为转前、转中 4h后每隔 2 h抽血标本 ;电镜标本为转前、转中每隔 4h抽一次。结果 :1、两组红细胞脆性度各时点的数值与其前一时点比较 ,没有差别。 2、电镜标本 :转前、转中 4、8、12、16 h标本中 ,每百个红细胞中棘球红细胞数 A,B两组分别为 :1.77± 0 .83,1.81± 0 .78;6 .12± 1.12 ,7.13± 1.2 5 ;9.18± 1.0 7,8.73±1.16 ;13.2 1± 1.32 ,12 .89± 1.5 3;16 .5 3± 1.2 4,17.2 1± 1.34。各时点与其前一时点值比较 ,有显著差异 (P <0 .0 5 )。两组均可见到各种变形红细胞。 3、两组游离血红蛋白 :随转流时间延长而呈线性不断增多。两组溶血指数分别为 0 .2 96 m g/ L / h和 0 .3993mg/ L / h。实验结果表明 :1、滚压泵长时间转流不会引起红细胞脆性度增加 ;2、滚压泵的挤压可致红细胞膜的直接损伤和破裂 ;3。
To observe the erythrocyte fragility and morphological changes of erythrocytes caused by roller pump. Ten tests were divided into two groups,Polystan pediatric pump group A (n=5)and COBE pump group B(n=5). Ten whole blood samples (each 400 ml) were circlulated in the roller pump for 16 h. Erythrocyte fragility and free hemoglobin were measured before pumping and at every 2 hours during pumping. The possible morphological changes of erythrocytes caused by roller pump were observed by scanning electron microscope. The electron microscopic observation was made before pumping and at every 4 hours throughout pumping. Results showed that the erythrocyte fragility of two groups was not increased during a long period of pumping. The number of acanthocytes of two groups was 1.77/1.81% in the samples before pumping and 6.12/7.13, 9.18/8.73, 13.21/12.89, 16.53/17.21% at 4 h, 8 h, 12 h,and 16 h respectively. The free hemoglobin level of two groups was increased linearly during a long duration of pumping and the index of hemolysis of two groups was 0.296 mg/L/h and 0.3993 mg/L/h respectively.The result shows: 1.the erythrocyte fragility was not increased during a long period of pumping; 2. the erythrocyte membrane was injured or broken by roller pump directely; 3.the morphological changes of erythrocytes would be the basis of post operative hemolysis.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
2002年第3期419-422,共4页
Journal of Biomedical Engineering
