摘要
【目的】改进体外成肌细胞培养方法。【方法】取 2 0只小鼠分 4组 ,取股骨肌用肌块法进行成肌细胞原代培养 ,并重复利用肌块 6次。对培养的成肌细胞进行生长状态观察及免疫的组化鉴定。【结果】重复 6次使用肌块培养的成肌细胞生长、分化良好 ;一次差速贴壁纯化后 ,经免疫组化方法鉴定 ,96 %以上的细胞呈肌细胞特异抗体阳性反应。【结论】重复肌块法培养成肌细胞 ,省略了重复组织获取、消化等烦琐步骤 ,培养效率明显提高 。
To improve the method of myoblast cell culture in vitro. 20 mice were equally divided into 4 groups. Myoblast cells were primary cultured with method of femur muscle pieces. These muscle pieces were reused for 6 times. The cultured cells were observed in growth state and by immunohistochemistry methods. The cultured myoblast cells used six times muscle pieces were differentiated and grew well. 96% myoblast cells, after purified one time with the method of different speed stick wall, were positively reacted to muscular particular antibody with immunohistochemistry method. [Conclusion] Myoblast culture with reused muscle pieces have the advantage of avoiding troubling procedures of acquiring and digesting muscle tissue, which can strikingly enhance the culture efficiency, and highly purified myoblast cells can be harvested by the method.
出处
《中山医科大学学报》
CSCD
北大核心
2002年第5期339-340,344,共3页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
国家自然科学基金资助项目 (3 0 170 3 3 7)
卫生部临床学科重点基金资助项目 (970 40 2 2 9
2 0 0 13 2 1)
教育部骨干教师基金资助项目 (2 0 0 0 2 3 49)
广东省卫生厅科研基金资助项目 (A2 0 0 0 14 9)
关键词
成肌细胞
细胞培养
重复肌块法
myoblast cell
cell culture
reuse muscullar pieces
